ArticleThe Biochemical journal1987
Production and characterization of monoclonal antibodies to insulin secretory granule membranes.
Article in The Biochemical journal, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.Molecular biology of the cell · 2012Article
- Role of furin in granular acidification in the endocrine pancreas: identification of the V-ATPase subunit Ac45 as a candidate substrate.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Lysosomes co-localize with ryanodine receptor subtype 3 to form a trigger zone for calcium signalling by NAADP in rat pulmonary arterial smooth muscle.Cell calcium · 2008Article
- The role of mVps18p in clustering, fusion, and intracellular localization of late endocytic organelles.Molecular biology of the cell · 2003Article
- Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion.Molecular biology of the cell · 2000Article
- Endolyn is a mucin-like type I membrane protein targeted to lysosomes by its cytoplasmic tail.The Biochemical journal · 2000Article
- Characterization of a fourth adaptor-related protein complex.Molecular biology of the cell · 1999Article
- Lumenal and transmembrane domains play a role in sorting type I membrane proteins on endocytic pathways.Molecular biology of the cell · 1998Article
- Apical plasma membrane proteins and endolyn-78 travel through a subapical compartment in polarized WIF-B hepatocytes.The Journal of cell biology · 1998Article
- Characterization of the adaptor-related protein complex, AP-3.The Journal of cell biology · 1997Article
- Endocytosis of activated TrkA: evidence that nerve growth factor induces formation of signaling endosomes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Delineation of the endocytic pathway of substance P and its seven-transmembrane domain NK1 receptor.Molecular biology of the cell · 1995Article
- Insulin secretory granule biogenesis and the proinsulin-processing endopeptidases.Diabetologia · 1994Review
- Sorting and processing of secretory proteins.The Biochemical journal · 1994Review
- Targeting and mistargeting of plasma membrane adaptors in vitro.The Journal of cell biology · 1993Article
- Low density lipoprotein receptor and cation-independent mannose 6-phosphate receptor are transported from the cell surface to the Golgi apparatus at equal rates in PC12 cells.The Journal of cell biology · 1992Article
- Glycoprotein II from adrenal chromaffin granules is also present in kidney lysosomes.The Biochemical journal · 1990Article
- The insulin secretory granule.Diabetologia · 1989Review
- Biosynthesis of insulin secretory granule membrane proteins. Control by glucose.The Biochemical journal · 1987Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monoclonal antibodies to insulin secretory granule membranes were obtained following immunization of mice with granule membranes purified from a rat transplantable insulinoma. The specificities of the antibodies were investigated by using binding assays with different insulinoma subcellular fractions, by indirect immunofluorescence studies with intact and permeabilized cells, and by immunoblotting of granule membrane proteins fractionated by SDS/polyacrylamide-gel electrophoresis. Fifty-six antibodies were characterized initially, and 21 representative cell lines were cloned. The antibodies fell into four categories: (1) binding preferentially to secretory granules, and reacting with a component of approx. 80,000 Da on immunoblots (antigen designated SGM 80); (2) binding preferentially to secretory granules, and reacting with components of approx. 110,000 and 50,000 Da on immunoblots (antigen designated SGM 110); (3) binding preferentially to secretory granules but unreactive on immunoblots; (4) binding to membrane antigen(s) with a widespread intracellular distribution which included granules and plasma membranes. The antigens SGM 80 and SGM 110 were studied in more detail and both were shown to be integral membrane glycoproteins with antigenic determinants located on the internal face of the secretory granule membrane. These antigens were also present in normal rat islets of Langerhans and similar components were detected by immunoblotting in secretory granules from anterior pituitary and adrenal medulla. Proteins which were immunologically related to SGM 80 and SGM 110, but distinct in molecular size, were also identified in liver. It is concluded that secretory granules contain specific components which are restricted in subcellular location but widespread in tissue distribution. The antibodies obtained will be valuable reagents in the further investigation of the biogenesis and turnover of insulin secretory granules.
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