ArticleNature methods2014
Objective comparison of particle tracking methods.
Abstract readComparative Study
In one paragraph
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 325 papers.
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325citing papers in PubMed
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325 citing papers in PubMed.
- OpticalFlow3D - a tool for measuring amorphous motion in three-dimensional fluorescence microscopy images.Journal of cell science · 2026Article
- Characterizing cellular receptor dynamics in live fluorescence imaging using stochastic modeling and machine learning.iScience · 2026Article
- Review of Machine Learning for Single-Particle Tracking: Methods, Challenges, and Biophysical Insights.Chemical & biomedical imaging · 2026Review
- Single-molecule localization and diffusivity microscopy reveals dynamic biomolecular organization in living cells.Nature methods · 2026Article
- Insulin receptor trafficking and interactions in muscle cells.Journal of the Endocrine Society · 2026Article
- FidlTrack: high-fidelity structure-aware single particle tracking resolves intracellular molecular motion in organelles sensing APP processing.Nature communications · 2026Article
- Bacteria break through one-micrometer-square passages by flagellar wrapping.Nature communications · 2026Article
- Artificial Neural Networks for Bioimage Analysis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- easytrack: A napari plugin for automated parameter tuning in cell tracking.microPublication biology · 2026Article
- Label-free TIMING: an efficient, reliable and scalable AI workflow for automated profiling of cell-cell interaction behaviors in nanowell arrays.Frontiers in bioinformatics · 2026Article
- Growth-rate analysis of clathrin assembly: inferring endocytic kinetics in living cells and tissues.Frontiers in molecular biosciences · 2026Review
- Revealing plasma membrane protein dynamics in living plant cells with single-molecule tracking.Journal of experimental botany · 2025Article
- ProTrack3D: a comprehensive tool for segmentation and tracking of proteins with split and fusion.BMC bioinformatics · 2025Article
- FloCyT: A Flow-Aware Centroid Tracker for Cell Analysis in High-Speed Capillary-Driven Microfluidic Flow.Sensors (Basel, Switzerland) · 2025Article
- Enhanced spatial clustering of single-molecule localizations with graph neural networks.Nature communications · 2025Article
- Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025Article
- Single-Photon Single-Particle Tracking.bioRxiv : the preprint server for biology · 2025Article
- Image analysis optimization for nanowire-based optical detection of molecules.Nanophotonics (Berlin, Germany) · 2025Article
- Pointwise prediction of protein diffusive properties using machine learning.JPhys photonics · 2025Article
- Quantitative evaluation of methods to analyze motion changes in single-particle experiments.Nature communications · 2025Article
265 more citing papers are in PubMed but not listed here.
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Authors and funding
35 authors.
Nicolas Chenouard1] Institut Pasteur, Unité d'Analyse d'Images Quantitative, Centre National de la Recherche Scientifique Unité de Recherche Associée 2582, Paris, France. [2] Biomedical Imaging Group, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [3] New York University Neuroscience Institute, New York University Medical Center, New York, New York, USA. [4].
Ihor Smal1] Department of Medical Informatics, Erasmus University Medical Center, Rotterdam, The Netherlands. [2] Department of Radiology, Erasmus University Medical Center, Rotterdam, The Netherlands. [3].
Fabrice de Chaumont1] Institut Pasteur, Unité d'Analyse d'Images Quantitative, Centre National de la Recherche Scientifique Unité de Recherche Associée 2582, Paris, France. [2].
Martin Maška1] Center for Applied Medical Research, University of Navarra, Pamplona, Spain. [2] Centre for Biomedical Image Analysis, Masaryk University, Brno, Czech Republic. [3].
Ivo F SbalzariniMOSAIC Group, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Yuanhao GongMOSAIC Group, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Janick CardinaleMOSAIC Group, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Craig CarthelCompunetix Inc., Monroeville, Pennsylvania, USA.
Stefano CoraluppiCompunetix Inc., Monroeville, Pennsylvania, USA.
Mark WinterDepartment of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania, USA.
Andrew R CohenDepartment of Electrical and Computer Engineering, Drexel University, Philadelphia, Pennsylvania, USA.
William J Godinez1] Department of Bioinformatics and Functional Genomics, Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany. [2] Division of Theoretical Bioinformatics, German Cancer Research Center, Heidelberg, Germany.
Karl Rohr1] Department of Bioinformatics and Functional Genomics, Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany. [2] Division of Theoretical Bioinformatics, German Cancer Research Center, Heidelberg, Germany.
Yannis Kalaidzidis1] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. [2] Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.
Liang LiangDepartment of Electrical Engineering, Yale University, New Haven, Connecticut, USA.
James DuncanDepartment of Electrical Engineering, Yale University, New Haven, Connecticut, USA.
Hongying ShenDepartment of Cell Biology, Yale University, New Haven, Connecticut, USA.
Yingke XuDepartment of Biomedical Engineering, Zhejiang University, Hangzhou, China.
Klas E G MagnussonDepartment of Signal Processing, ACCESS Linnaeus Centre, KTH Royal Institute of Technology, Stockholm, Sweden.
Joakim JaldénDepartment of Signal Processing, ACCESS Linnaeus Centre, KTH Royal Institute of Technology, Stockholm, Sweden.
Helen M BlauBaxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
Perrine Paul-GilloteauxCell and Tissue Imaging Facility, Institut Curie, Paris, France.ORCID 0000000339034841
Philippe RoudotInria Rennes, Bretagne Atlantique, Rennes, France.
Charles KervrannInria Rennes, Bretagne Atlantique, Rennes, France.
François WaharteCell and Tissue Imaging Facility, Institut Curie, Paris, France.
Jean-Yves TinevezPlateforme d'Imagerie Dynamique, Imagopole, Institut Pasteur, Paris, France.
Spencer L ShortePlateforme d'Imagerie Dynamique, Imagopole, Institut Pasteur, Paris, France.
Joost WillemseMolecular Biotechnology Group, Institute of Biology, Leiden University, Leiden, The Netherlands.
Katherine CellerMolecular Biotechnology Group, Institute of Biology, Leiden University, Leiden, The Netherlands.
Gilles P van WezelMolecular Biotechnology Group, Institute of Biology, Leiden University, Leiden, The Netherlands.ORCID 0000000303411561
Han-Wei DanDepartment of Biomedical Engineering, Chung Yuan Christian University, Chung Li City, Taiwan, China.
Yuh-Show TsaiDepartment of Biomedical Engineering, Chung Yuan Christian University, Chung Li City, Taiwan, China.
Carlos Ortiz de SolórzanoCenter for Applied Medical Research, University of Navarra, Pamplona, Spain.
Jean-Christophe Olivo-Marin1] Institut Pasteur, Unité d'Analyse d'Images Quantitative, Centre National de la Recherche Scientifique Unité de Recherche Associée 2582, Paris, France. [2].
Erik Meijering1] Department of Medical Informatics, Erasmus University Medical Center, Rotterdam, The Netherlands. [2] Department of Radiology, Erasmus University Medical Center, Rotterdam, The Netherlands. [3].
Funding
Basic and Translational Research iPSC-based hematologic and vascular therapiesU01HL100397 · NHLBI · STANFORD UNIVERSITY · PI COOKE, JOHN P · 2009 to 2015
$7.4MNeuronal Adaptation and Plasticity after Chronic DisuseR01MH071739 · NIMH · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI TSIEN, RICHARD W · 2015 to 2024
$5.5MStem Cells for Brain and BrawnR01AG020961 · NIA · STANFORD UNIVERSITY · PI BLAU, HELEN M · 2002 to 2019
$5.0MACTIVATORS OF MUSCLE GENESR01AG009521 · NIA · STANFORD UNIVERSITY · PI BLAU, HELEN M · 1990 to 2014
$4.7MVesicular Retrieval and Reuse at CNS Nerve TerminalsR01MH064070 · NIMH · STANFORD UNIVERSITY · PI TSIEN, RICHARD W · 2001 to 2010
$3.9MSynaptic Adaptation and Plasticity After Chronic DisuseR37MH071739 · NIMH · STANFORD UNIVERSITY · PI TSIEN, RICHARD W · 2004 to 2013
$3.8MRegulation of Hematopoietic Stem Cell Fate in Bioengineered NichesR01HL096113 · NHLBI · STANFORD UNIVERSITY · PI BLAU, HELEN M · 2009 to 2013
$2.7MAnalyzing Neural Stem Cell Clonal DevelopmentR01NS076709 · NINDS · UNIVERSITY OF WISCONSIN MILWAUKEE · PI COHEN, ANDREW ROBERT · 2011 to 2015
$1.7MVesicular Retrieval and Reuse at CNS Nerve TerminalsR56MH064070 · NIMH · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI TSIEN, RICHARD W · 2011 to 2012
$841k3D Bioengineering Strategies to Mimic Human Skeletal Muscle Progenitor Cell NicheR21AR062359 · NIAMS · STANFORD UNIVERSITY · PI BLAU, HELEN M, HEILSHORN, SARAH C · 2011 to 2012
$367kNHLBI NIH HHS R01 HL096113NHLBI NIH HHS U01 HL100397NIAMS NIH HHS R21 AR062359NIA NIH HHS R01 AG009521NIA NIH HHS R01 AG020961NIMH NIH HHS MH064070NIMH NIH HHS MH071739NIMH NIH HHS R01 MH064070NIMH NIH HHS R01 MH071739NIMH NIH HHS R37 MH071739NIMH NIH HHS R56 MH064070NINDS NIH HHS R01 NS076709NINDS NIH HHS R01NS076709
Abstract
Particle tracking is of key importance for quantitative analysis of intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting and following large numbers of individual particles is not feasible, automated computational methods have been developed for these tasks by many groups. Aiming to perform an objective comparison of methods, we gathered the community and organized an open competition in which participating teams applied their own methods independently to a commonly defined data set including diverse scenarios. Performance was assessed using commonly defined measures. Although no single method performed best across all scenarios, the results revealed clear differences between the various approaches, leading to notable practical conclusions for users and developers.
Indexed as
Image Interpretation, Computer-AssistedMicroscopy, Fluorescence
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LicenceCC BY-NC-SA
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.