Evidence map›Paper›PMID 24441880›Full record

ArticleNature materials2014

Transformation of taxol-stabilized microtubules into inverted tubulin tubules triggered by a tubulin conformation switch.

Miguel A Ojeda-Lopez, Daniel J Needleman, Chaeyeon Song, Avi Ginsburg, Phillip A Kohl, Youli Li, Herbert P Miller, Leslie Wilson, Uri Raviv, Myung Chul Choi and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature materials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Review
  15. Article
  16. Review
  17. Journal of applied crystallography · 2019
    Article
  18. Progress in research on paclitaxel and tumor immunotherapy.Cellular & molecular biology letters · 2019
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Miguel A Ojeda-Lopez *Materials Department, Physics Department, Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA.
Daniel J Needleman *Materials Department, Physics Department, Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA.
Chaeyeon Song *Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
Avi GinsburgInstitute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, 91904 Jerusalem, Israel.
Phillip A KohlMaterials Research Laboratory, University of California, Santa Barbara, CA 93106, USA.
Youli LiMaterials Research Laboratory, University of California, Santa Barbara, CA 93106, USA.
Herbert P MillerMolecular, Cellular, & Developmental Biology Department & Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA.
Leslie WilsonMolecular, Cellular, & Developmental Biology Department & Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA.
Uri RavivInstitute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, 91904 Jerusalem, Israel.
Myung Chul ChoiDepartment of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
Cyrus R SafinyaMaterials Department, Physics Department, Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA 93106, USA.

Funding

Structures of Lipid-DNA Nonviral Gene Delivery Systems 4R01GM059288 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI SAFINYA, CYRUS R · 1999 to 2016
$4.2M
Mechanism &Control of Brain Microtubule DynamicsR01NS013560 · NINDS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI WILSON, LESLIE · 1985 to 2009
$2.9M
NIGMS NIH HHS R01 GM059288NINDS NIH HHS R01 NS013560NINDS NIH HHS R01-NS13560
6 · The paper itself

Abstract

Bundles of taxol-stabilized microtubules (MTs)--hollow tubules comprised of assembled αβ-tubulin heterodimers--spontaneously assemble above a critical concentration of tetravalent spermine and are stable over long times at room temperature. Here we report that at concentrations of spermine several-fold higher the MT bundles (B(MT)) quickly become unstable and undergo a shape transformation to bundles of inverted tubulin tubules (B(ITT)), the outside surface of which corresponds to the inner surface of the B(MT) tubules. Using transmission electron microscopy and synchrotron small-angle X-ray scattering, we quantitatively determined both the nature of the B(MT)-to-B(ITT) transformation pathway, which results from a spermine-triggered conformation switch from straight to curved in the constituent taxol-stabilized tubulin oligomers, and the structure of the B(ITT) phase, which is formed of tubules of helical tubulin oligomers. Inverted tubulin tubules provide a platform for studies requiring exposure and availability of the inside, luminal surface of MTs to MT-targeted drugs and MT-associated proteins.

Indexed as

AnimalsBinding SitesCattleMicrotubulesPaclitaxelProtein BindingTubulinPaclitaxelTubulin

Identifiers

PMID24441880
PMCPMC3946914

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.