ArticleNature biotechnology2014
Single-molecule site-specific detection of protein phosphorylation with a nanopore.
Article in Nature biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
110 citing papers in PubMed.
- Site-specific post-translational modification detection by polar charged engineered MspA nanopores.Chemical science · 2026Article
- Nanopore Fingerprinting of Neurodegenerative Proteins and Phosphoproteins within a Minute.JACS Au · 2026Article
- Nanopore-based massively parallel sensing for peptide profiling and protein identification.Nature communications · 2026Article
- Review
- Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optical and Electrical Characterization of Nanopores in Freestanding Lipid Bilayers Formed on Microelectrode Cavity Arrays.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Altered glycosylation of EVs in cancers: galectin-3-binding protein (LGALS3BP).Discover oncology · 2025Review
- Eliminating the Interference of Neighboring Nucleobases in Aerolysin for Nanopore Sequencing.ACS sensors · 2025Article
- Single-molecule nanopore sensing of prolineChemical science · 2025Article
- Single-Molecule SERS Discrimination of Proline from Hydroxyproline Assisted by a Deep Learning Model.Nano letters · 2025Article
- Direct mapping of tyrosine sulfation states in native peptides by nanopore.Nature chemical biology · 2025Article
- Nanopore sensing of protein and peptide conformation for point-of-care applications.Nature communications · 2025Review
- Toward single-molecule protein sequencing using nanopores.Nature biotechnology · 2025Review
- Nanopore approaches for single-molecule temporal omics: promises and challenges.Nature methods · 2025Review
- Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules.Biosensors · 2024Review
- Location of Phosphorylation Sites within Long Polypeptide Chains by Binder-Assisted Nanopore Detection.Journal of the American Chemical Society · 2024Article
- Article
- Label-free electronic detection of peptide post-translational modification with functional enzyme-driven assay at the physical limit.Cell reports. Physical science · 2024Article
- Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force.Nature biotechnology · 2024Article
- Single-Molecule Electrical Profiling of Peptides and Proteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We demonstrate single-molecule, site-specific detection of protein phosphorylation with protein nanopore technology. A model protein, thioredoxin, was phosphorylated at two adjacent sites. Analysis of the ionic current amplitude and noise, as the protein unfolds and moves through an α-hemolysin pore, enables the distinction between unphosphorylated, monophosphorylated and diphosphorylated variants. Our results provide a step toward nanopore proteomics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.