ArticleEuropean journal of histochemistry : EJH2013
Three-dimensional scaffold-free fusion culture: the way to enhance chondrogenesis of in vitro propagated human articular chondrocytes.
Article in European journal of histochemistry : EJH, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Engineering Multi-Spheroid Bone Constructs Through the Fusion of Large MSC Spheroids.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- From microtissues to macro solutions - The future of scalable and automated cartilage tissue engineering.Journal of orthopaedic translation · 2026Review
- Injectable MSC Spheroid and Microgel Granular Composites for Engineering Tissue.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Temporal Enzymatic Treatment to Enhance the Remodeling of Multiple Cartilage Microtissues into a Structurally Organized Tissue.Advanced healthcare materials · 2024Article
- Hyperplastic Human Macromass Cartilage for Joint Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Articular Cartilage Regeneration through Bioassembling Spherical Micro-Cartilage Building Blocks.Cells · 2022Review
- Spheroid-Based Tissue Engineering Strategies for Regeneration of the Intervertebral Disc.International journal of molecular sciences · 2022Review
- The role of TGF-β2 in cartilage development and diseases.Bone & joint research · 2021Article
- Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications.Biomedicines · 2021Article
- Three-dimensional, Scaffold-Free, Autologous Chondrocyte Transplantation: A Systematic Review.Orthopaedic journal of sports medicine · 2020Review
- Cartilage and bone tissue engineering using adipose stromal/stem cells spheroids as building blocks.World journal of stem cells · 2020Review
- Impact of Hydrogel Stiffness on Differentiation of Human Adipose-Derived Stem Cell Microspheroids.Tissue engineering. Part A · 2019Article
- Tissue Specific Differentiation of Human Chondrocytes Depends on Cell Microenvironment and Serum Selection.Cells · 2019Article
- Dynamic formation of cellular aggregates of chondrocytes and mesenchymal stem cells in spinner flask.Cell proliferation · 2019Article
- Development of three-dimensional articular cartilage construct using silica nano-patterned substrate.PloS one · 2019Article
- Testing the potency of anti-TNF-α and anti-IL-1β drugs using spheroid cultures of human osteoarthritic chondrocytes and donor-matched chondrogenically differentiated mesenchymal stem cells.Biotechnology progress · 2018Article
- Article
- Review
- Featured Article: In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes.Experimental biology and medicine (Maywood, N.J.) · 2017Article
- High seeding density of human chondrocytes in agarose produces tissue-engineered cartilage approaching native mechanical and biochemical properties.Journal of biomechanics · 2016Article
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6 authors.
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Abstract
Cartilage regeneration based on isolated and culture-expanded chondrocytes has been studied in various in vitro models, but the quality varies with respect to the morphology and the physiology of the synthesized tissues. The aim of our study was to promote in vitro chondrogenesis of human articular chondrocytes using a novel three-dimensional (3-D) cultivation system in combination with the chondrogenic differentiation factors transforming growth factor beta 2 (TGF-b2) and L-ascorbic acid. Articular chondrocytes isolated from six elderly patients were expanded in monolayer culture. A single-cell suspension of the dedifferentiated chondrocytes was then added to agar-coated dishes without using any scaffold material, in the presence, or absence of TGF-b2 and/or L-ascorbic acid. Three-dimensional cartilage-like constructs, called single spheroids, and microtissues consisting of several spheroids fused together, named as fusions, were formed. Generated tissues were mainly characterized using histological and immunohistochemical techniques. The morphology of the in vitro tissues shared some similarities to native hyaline cartilage in regard to differentiated S100-positive chondrocytes within a cartilaginous matrix, with strong collagen type II expression and increased synthesis of proteoglycans. Finally, our innovative scaffold-free fusion culture technique supported enhanced chondrogenesis of human articular chondrocytes in vitro. These 3-D hyaline cartilage-like microtissues will be useful for in vitro studies of cartilage differentiation and regeneration, enabling optimization of functional tissue engineering and possibly contributing to the development of new approaches to treat traumatic cartilage defects or osteoarthritis.
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