Evidence map›Paper›PMID 24440664›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2014

Lung gene therapy with highly compacted DNA nanoparticles that overcome the mucus barrier.

Jung Soo Suk, Anthony J Kim, Kanika Trehan, Craig S Schneider, Liudmila Cebotaru, Owen M Woodward, Nicholas J Boylan, Michael P Boyle, Samuel K Lai, William B Guggino and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.

0numbers the graph read from it
0cells of the map it votes in
86citing papers in PubMed
14.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

86 citing papers in PubMed, 189 citations in OpenAlex.

  1. Chemically modified CRISPR enzymes for multi-organ genome editingbioRxiv : the preprint server for biology · 2026
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  10. pH modification of gel mobility shift improves polyplex selection In Vivo.Biochemical and biophysical research communications · 2024
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  20. Mechanisms and challenges of nanocarriers as non-viral vectors of therapeutic genes for enhanced pulmonary delivery.Journal of controlled release : official journal of the Controlled Release Society · 2022
    Review

26 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Jung Soo SukThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Ophthalmology, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore 21205, USA.
Anthony J KimThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA.
Kanika TrehanThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Molecular & Cellular Biology, The Johns Hopkins University, 3400 North Charles Street, Baltimore 21218, USA.
Craig S SchneiderThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Chemical & Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore 21218, USA.
Liudmila CebotaruThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Ophthalmology, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA.
Owen M WoodwardDepartment of Physiology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore 21205, USA.
Nicholas J BoylanDepartment of Chemical & Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore 21218, USA.
Michael P BoyleJohns Hopkins Adult Cystic Fibrosis Program, Division of Pulmonary and Critical Care Medicine, The Johns Hopkins University School of Medicine, 1830 East Monument Street, Baltimore 21205, USA.
Samuel K LaiThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Chemical & Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore 21218, USA.
William B GugginoThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Physiology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore 21205, USA.
Justin HanesThe Center for Nanomedicine, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Ophthalmology, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore 21231, USA; Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore 21205, USA; Department of Chemical & Biomolecular Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore 21218, USA. Electronic address: hanes@jhmi.edu.
Johns Hopkins University · USJohns Hopkins Medicine · US

Funding

Vector CoreP01HL051811 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HANES, JUSTIN S. · 1993 to 2013
$20.2M
New Gene Vectors for Cystic Fibrosis (CF): Extra- & Intracellular BarriersR01EB003558 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI HANES, JUSTIN S. · 2007 to 2010
$1.4M
Design of Non-viral Gene Carriers that Overcome Extra- and Intracellular BarriersF32HL103137 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI KIM, ANTHONY J. · 2011 to 2012
$102k
NHLBI NIH HHS F32 HL103137NHLBI NIH HHS P01 HL051811NHLBI NIH HHS P01 HL51811NIBIB NIH HHS R01 EB003558NIBIB NIH HHS R01EB003558
6 · The paper itself

Abstract

Inhaled gene carriers must penetrate the highly viscoelastic and adhesive mucus barrier in the airway in order to overcome rapid mucociliary clearance and reach the underlying epithelium; however, even the most widely used viral gene carriers are unable to efficiently do so. We developed two polymeric gene carriers that compact plasmid DNA into small and highly stable nanoparticles with dense polyethylene glycol (PEG) surface coatings. These highly compacted, densely PEG-coated DNA nanoparticles rapidly penetrate human cystic fibrosis (CF) mucus ex vivo and mouse airway mucus ex situ. Intranasal administration of the mucus penetrating DNA nanoparticles greatly enhanced particle distribution, retention and gene transfer in the mouse lung airways compared to conventional gene carriers. Successful delivery of a full-length plasmid encoding the cystic fibrosis transmembrane conductance regulator protein was achieved in the mouse lungs and airway cells, including a primary culture of mucus-covered human airway epithelium grown at air-liquid interface, without causing acute inflammation or toxicity. Highly compacted mucus penetrating DNA nanoparticles hold promise for lung gene therapy.

Indexed as

Gene Transfer TechniquesAdministration, IntranasalAdultAnimalsCHO CellsCricetulusCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDNAEsophagusFemaleGastric MucosaGenetic TherapyHumansLungMice, Inbred BALB CCystic Fibrosis Transmembrane Conductance RegulatorDNAPolyethylene GlycolsPolyethyleneiminePolylysineAirway gene therapyDNA nanoparticleMucus barrierMultiple particle tracking

Identifiers

PMID24440664
PMCPMC3951606
OpenAlexW2020266608

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.