Evidence map›Paper›PMID 24440645›Full record

ArticleClinical immunology (Orlando, Fla.)2014

SLAP deficiency decreases dsDNA autoantibody production.

Lisa K Peterson, Luke F Pennington, Laura A Shaw, Meredith Brown, Eric C Treacy, Samantha F Friend, Øyvind Hatlevik, Kira Rubtsova, Anatoly V Rubtsov, Leonard L Dragone

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical immunology (Orlando, Fla.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Lisa K PetersonDepartment of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Luke F PenningtonDepartment of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Laura A ShawDepartment of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Meredith BrownDepartment of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Eric C TreacyDepartment of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Samantha F FriendDepartment of Pediatrics, University of Colorado Denver, 13001 E. 17th Place, Aurora, CO 80045, USA; Department of Immunology, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Øyvind HatlevikDepartment of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Kira RubtsovaDepartment of Immunology, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Anatoly V RubtsovDepartment of Immunology, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.
Leonard L DragoneDepartment of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA; Department of Pediatrics, University of Colorado Denver, 13001 E. 17th Place, Aurora, CO 80045, USA; Department of Immunology, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA; Division of Rheumatology, Colorado Children's Hospital, 13123 E. 16th Ave., Aurora, CO 80045, USA. Electronic address: dragonel@njhealth.org.
National Jewish Health · USUniversity of Colorado Denver · USChildren's Hospital Colorado · US

Funding

Medical Scientist Training ProgramT32GM008497 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI WILSON, CARA C. · 1993 to 2022
$15.2M
Research Training in Rheumatology INCLUDE Down Syndrome SupplementT32AR007534 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI Kristine A. Kuhn · 1986 to 2026
$6.1M
Molecular Mechanisms of Immune ToleranceT32AI074491 · NIAID · NATIONAL JEWISH HEALTH · PI CAMBIER, JOHN C, PELANDA, ROBERTA · 2009 to 2023
$4.1M
Arthritis Research UKNIAID NIH HHS T32 AI074491NIAMS NIH HHS T32 AR007534NIGMS NIH HHS T32 GM008497
6 · The paper itself

Abstract

Src-like adaptor protein (SLAP) adapts c-Cbl, an E3 ubiquitin ligase, to activated components of the BCR signaling complex regulating BCR levels and signaling in developing B cells. Based on this function, we asked whether SLAP deficiency could decrease the threshold for tolerance and eliminate development of autoreactive B cells in two models of autoantibody production. First, we sensitized mice with a dsDNA mimetope that causes an anti-dsDNA response. Despite equivalent production of anti-peptide antibodies compared to BALB/c controls, SLAP(-/-) mice did not produce anti-dsDNA. Second, we used the 56R tolerance model. SLAP(-/-) 56R mice had decreased levels of dsDNA-reactive antibodies compared to 56R mice due to skewed light chain usage. Thus, SLAP is a critical regulator of B-cell development and function and its deficiency leads to decreased autoreactive B cells that are otherwise maintained by inefficient receptor editing or failed negative selection.

Indexed as

AnimalsAntibodies, AntinuclearB-Lymphocyte SubsetsDNAFemaleImmunoglobulin Heavy ChainsImmunoglobulin kappa-ChainsImmunoglobulin lambda-ChainsMiceMice, KnockoutProto-Oncogene Proteins pp60(c-src)Receptors, Antigen, B-CellAntibodies, AntinuclearDNAImmunoglobulin Heavy ChainsImmunoglobulin kappa-ChainsImmunoglobulin lambda-ChainsProto-Oncogene Proteins pp60(c-src)Receptors, Antigen, B-CellSla protein, mouseAutoantibodiesAutoimmunityB cells

Identifiers

PMID24440645
PMCPMC4053206
OpenAlexW1999142426

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.