ArticleArchives of biochemistry and biophysics2014
Candidate mediators of chondrocyte mechanotransduction via targeted and untargeted metabolomic measurements.
Article in Archives of biochemistry and biophysics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- The metabolome of male and female individuals with knee osteoarthritis is influenced by 18-months of weight loss intervention: the IDEA trial.BMC musculoskeletal disorders · 2024Trial
- Viscoelastic recovery time of chondrocytes from monolayer and alginate cultures.bioRxiv : the preprint server for biology · 2026Article
- Chondrocytes Embedded in Agarose Generate Distinct Metabolic Heat Profiles Based on Media Carbon Sources.Annals of biomedical engineering · 2025Article
- Metabolic Profiles of Encapsulated Chondrocytes Exposed to Short-Term Simulated Microgravity.Annals of biomedical engineering · 2025Article
- The metabolome of individuals with knee osteoarthritis is influenced by 18-months of an exercise and weight loss intervention and sex: the IDEA trial.bioRxiv : the preprint server for biology · 2024Article
- Germ-Free C57BL/6 Mice Have Increased Bone Mass and Altered Matrix Properties but Not Decreased Bone Fracture Resistance.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Metabolomic Profiling to Understand Chondrocyte Metabolism.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Correlations between metabolites in the synovial fluid and serum: A mouse injury study.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Article
- Metabolomic Profiling and Mechanotransduction of Single Chondrocytes Encapsulated in Alginate Microgels.Cells · 2022Article
- Mechanosignalling in cartilage: an emerging target for the treatment of osteoarthritis.Nature reviews. Rheumatology · 2022Review
- Effects of mechanical stimulation on metabolomic profiles of SW1353 chondrocytes: shear and compression.Biology open · 2022Article
- [Research Progress in Glucose Metabolism of Chondrocytes].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2021Review
- Primary human chondrocytes respond to compression with phosphoproteomic signatures that include microtubule activation.Journal of biomechanics · 2019Article
- Inhibition of early response genes prevents changes in global joint metabolomic profiles in mouse post-traumatic osteoarthritis.Osteoarthritis and cartilage · 2019Article
- Physiological dynamic compression regulates central energy metabolism in primary human chondrocytes.Biomechanics and modeling in mechanobiology · 2019Article
- Metabolic responses induced by compression of chondrocytes in variable-stiffness microenvironments.Journal of biomechanics · 2017Article
- Combining Targeted Metabolomic Data with a Model of Glucose Metabolism: Toward Progress in Chondrocyte Mechanotransduction.PloS one · 2017Article
- Emerging role of metabolic signaling in synovial joint remodeling and osteoarthritis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2016Review
- Mechanotransduction in primary human osteoarthritic chondrocytes is mediated by metabolism of energy, lipids, and amino acids.Journal of biomechanics · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Chondrocyte mechanotransduction is the process by which cartilage cells transduce mechanical loads into biochemical and biological signals. Previous studies have identified several pathways by which chondrocytes transduce mechanical loads, yet a general understanding of which signals are activated and in what order remains elusive. This study was performed to identify candidate mediators of chondrocyte mechanotransduction using SW1353 chondrocytes embedded in physiologically stiff agarose. Dynamic compression was applied to cell-seeded constructs for 0-30min, followed immediately by whole-cell metabolite extraction. Metabolites were detected via LC-MS, and compounds of interest were identified via database searches. We found several metabolites which were statistically different between the experimental groups, and we report the detection of 5 molecules which are not found in metabolite databases of known compounds indicating potential novel molecules. Targeted studies to quantify the response of central energy metabolites to compression found a transient increase in the ratio of NADP+ to NADPH and a continual decrease in the ratio of GDP to GTP, suggesting a flux of energy into the TCA cycle. These data are consistent with the remodeling of cytoskeletal components by mechanically induced signaling, and add substantial new data to a complex picture of how chondrocytes transduce mechanical loads.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.