ArticleExperimental eye research2014
Corneal stromal stem cells versus corneal fibroblasts in generating structurally appropriate corneal stromal tissue.
Article in Experimental eye research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 82 citations in OpenAlex.
- Co-differentiation and enrichment of corneal endothelial cells and keratocytes from human pluripotent stem cells.Scientific reports · 2025Article
- Review
- Cell sheet-based approach to study the diabetic corneal stroma.Experimental eye research · 2023Article
- Corneal stromal regeneration-keratoconus cell therapy: a review.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2023Review
- Profile of biological characterizations and clinical application of corneal stem/progenitor cells.World journal of stem cells · 2022Review
- Regenerative therapy for the Cornea.Progress in retinal and eye research · 2022Review
- Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy.Cells · 2022Article
- Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma.Scientific reports · 2021Article
- Type 2 immunity induced by bladder extracellular matrix enhances corneal wound healing.Science advances · 2021Article
- In vitro reconstructed 3D corneal tissue models for ocular toxicology and ophthalmic drug development.In vitro cellular & developmental biology. Animal · 2021Review
- The Human Tissue-Engineered Cornea (hTEC): Recent Progress.International journal of molecular sciences · 2021Review
- Corneal stromal mesenchymal stem cells: reconstructing a bioactive cornea and repairing the corneal limbus and stromal microenvironment.International journal of ophthalmology · 2021Review
- Article
- Effect of porcine corneal stromal extract on keratocytes from SMILE-derived lenticules.Journal of cellular and molecular medicine · 2021Article
- Fibroblastic and bone marrow-derived cellularity in the corneal stroma.Experimental eye research · 2021Article
- Induction of Irritation and Inflammation in a 3D Innervated Tissue Model of the Human Cornea.ACS biomaterials science & engineering · 2020Article
- Methods for Investigating Corneal Cell Interactions and Extracellular Vesicles In Vitro.Current protocols in cell biology · 2020Article
- The anti-scarring effect of corneal stromal stem cell therapy is mediated by transforming growth factor β3.Eye and vision (London, England) · 2020Article
- Engineering a Corneal Stromal Equivalent Using a Novel Multilayered Fabrication Assembly Technique.Tissue engineering. Part A · 2020Article
- Corneal stromal stem cells restore transparency after NStem cells translational medicine · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recapitulation of human corneal stromal tissue is believed to be among the most challenging steps in engineering human corneal tissue because of the difficulty in reproducing its highly-ordered hierarchical ultrastructure, which imparts its robust biomechanical properties and optical transparency. In this study, we compared the feasibility of utilizing human corneal stromal stem cells (hCSSCs) and human corneal fibroblasts (hCFs) in the generation of human corneal stromal tissue on a highly-aligned fibrous substrate made from poly(ester urethane) urea. In the serum-free keratocyte differentiation medium supplemented with FGF-2 (10 ng/mL) and TGF-β3 (0.1 ng/mL), hCSSCs successfully differentiated into keratocytes and secreted multilayered lamellae with orthogonally-oriented collagen fibrils, in a pattern mimicking human corneal stromal tissue. The constructs were 60-70 μm thick and abundant in cornea-specific extracellular matrix (ECM) components, including keratan sulfate, lumican, and keratocan. Under the identical conditions, hCFs tended to differentiate into myofibroblasts and deposited a less-organized collagen-fibrillar construct in a pattern with similarities to corneal scar tissue due to a lack of cornea-specific ECM components. These observations demonstrated that hCSSCs showed a much greater potential, under proper substrate and growth factor guidance, to facilitate the generation of a biological human cornea equivalent. Unlike hCSSCs, hCFs were less responsive to these environmental cues and under identical culture conditions generated an ECM that poorly mimicked the native, functional tissue structure and composition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.