ArticleChemistry & biology2014
Detection of miRNA expression in intact cells using activatable sensor oligonucleotides.
Article in Chemistry & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Peritumoral Microenvironment in High-Grade Gliomas: From FLAIRectomy to Microglia-Glioma Cross-Talk.Brain sciences · 2021Review
- MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer.Scientific reports · 2021Article
- Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes.Chemical Society reviews · 2017Review
- A conformation-induced fluorescence method for microRNA detection.Nucleic acids research · 2016Article
- In Vivo Detection of miRNA Expression in Tumors Using an Activatable Nanosensor.Molecular imaging and biology · 2016Article
- Emerging Biosensing Approaches for microRNA Analysis.Analytical chemistry · 2016Review
- Tiny giants of gene regulation: experimental strategies for microRNA functional studies.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
We describe a technology for the profiling of miRNA expression in intact cells. The technology is based on sensor oligonucleotides that are cleavable, completely complementary to a target miRNA, and dual-labeled with a fluorescent dye and a quencher. Upon entering the cell, the sensor oligonucleotide binds its specific miRNA target through complementary base-pairing. This triggers assembly of the endogenous RNA Induced Silencing Complex (RISC) around the miRNA-sensor duplex and cleavage of the sensor oligonucleotide, resulting in separation between the dye and quencher, and a fluorescence turn-on. In the presented feasibility studies, we focus on a specific miRNA (miR-10b) implicated in breast cancer metastasis. Using a human breast adenocarcinoma cell line, we illustrate the application of this technology for miRNA detection with nanomolar sensitivity in both a cell-free system and intact cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.