ArticleBiomaterials2014
Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Acellular embryoid body and hydroxybutyl chitosan composite hydrogels promote M2 macrophage polarization and accelerate diabetic cutaneous wound healing.Materials today. Bio · 2024Article
- Generation of bovine decellularized testicular bio-scaffolds as a 3D platform for testis bioengineering.Frontiers in bioengineering and biotechnology · 2024Article
- O-alg-THAM/gel hydrogels functionalized with engineered microspheres based on mesenchymal stem cell secretion recruit endogenous stem cells for cartilage repair.Bioactive materials · 2023Article
- A Review of Stem Cell Technology Targeting Hepatocyte Growth as an Alternative to Organ Transplantation.Methods in molecular biology (Clifton, N.J.) · 2023Review
- The ECM: To Scaffold, or Not to Scaffold, That Is the Question.International journal of molecular sciences · 2021Review
- Article
- Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bioscaffold.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Whole-ovary decellularization generates an effective 3D bioscaffold for ovarian bioengineering.Journal of assisted reproduction and genetics · 2020Article
- Functional role of glycosaminoglycans in decellularized lung extracellular matrix.Acta biomaterialia · 2020Article
- Lung Microvascular Niche, Repair, and Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Laminin-driven Epac/Rap1 regulation of epithelial barriers on decellularized matrix.Acta biomaterialia · 2019Article
- Re-endothelialization of rat lung scaffolds through passive, gravity-driven seeding of segment-specific pulmonary endothelial cells.Journal of tissue engineering and regenerative medicine · 2018Article
- An Official American Thoracic Society Workshop Report 2015. Stem Cells and Cell Therapies in Lung Biology and Diseases.Annals of the American Thoracic Society · 2016Article
- Enhanced Re-Endothelialization of Decellularized Rat Lungs.Tissue engineering. Part C, Methods · 2016Article
- Revascularization of decellularized lung scaffolds: principles and progress.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- An official American Thoracic Society workshop report: stem cells and cell therapies in lung biology and diseases.Annals of the American Thoracic Society · 2015Article
- Hypoxia enhances differentiation of mouse embryonic stem cells into definitive endoderm and distal lung cells.Stem cells and development · 2015Article
- Stem Cells and Regenerative Medicine: Myth or Reality of the 21th Century.Stem cells international · 2015Review
- Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture.PloS one · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Repopulation of decellularized lung scaffolds (DLS) is limited due to alterations in the repertoire and ratios of the residual extracellular matrix (ECM) proteins, characterized by e.g., the retention of type I collagen and loss of glycoproteins. We hypothesized that pre-treatment of decellularized matrices with defined ECM proteins, which match the repertoire of integrin receptors expressed by the cells to be seeded (e.g., embryonic stem cells) can increase the efficacy of the reseeding process. To test this hypothesis, we first determined the integrin receptors profile of mouse embryonic stem cells (mESCs). Mouse ESCs express α3, α5, α6, α9 and β1, but not α1, α2 and α4 integrin subunits, as established by Western blotting and adhesion to laminin and fibronectin, but not to collagens type I and IV. Reseeding of DLS with mESCs was inefficient (6.9 ± 0.5%), but was significantly enhanced (2.3 ± 0.1 fold) by pre-treating the scaffolds with media conditioned by A549 human lung adenocarcinoma cells, which we found to contain ∼5 μg/ml laminin. Furthermore, pre-treatment with A549-conditioned media resulted in a significantly more uniform distribution of the seeded mESCs throughout the engineered organ as compared to untreated DLS. Our study may advance whole lung engineering by stressing the importance of matching the integrin receptor repertoire of the seeded cells and the cell binding motifs of DLS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.