Evidence map›Paper›PMID 24439403›Full record

ArticleBiomaterials2014

The effect of side-chain functionality and hydrophobicity on the gene delivery capabilities of cationic helical polypeptides.

Rujing Zhang, Nan Zheng, Ziyuan Song, Lichen Yin, Jianjun Cheng

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 101 citations in OpenAlex.

  1. Review
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  15. Production and clinical development of nanoparticles for gene delivery.Molecular therapy. Methods & clinical development · 2016
    Review
  16. Article
  17. In vitro selection of a sodium-specific DNAzyme and its application in intracellular sensing.Proceedings of the National Academy of Sciences of the United States of America · 2015
    Article
  18. Ionic α-helical polypeptides toward nonviral gene delivery.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Rujing ZhangDepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL 61801, USA.
Nan ZhengDepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL 61801, USA.
Ziyuan SongDepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL 61801, USA.
Lichen YinDepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL 61801, USA. Electronic address: lcyin@illinois.edu.
Jianjun ChengDepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W Green Street, Urbana, IL 61801, USA. Electronic address: jianjunc@illinois.edu.
University of Illinois Urbana-Champaign · US

Funding

Developing Clinically Applicable, Cancer-Targeting Polymeric NanoconjugatesDP2OD007246 · OD · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI CHENG, JIANJUN · 2010 to 2010
$2.4M
Development of Helical Polypeptides for Non-Viral Gene Delivery SystemsR21EB013379 · NIBIB · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI CHENG, JIANJUN · 2011 to 2012
$415k
NIBIB NIH HHS 1R21EB013379NIBIB NIH HHS R21 EB013379NIH HHS 1DP2OD007246NIH HHS DP2 OD007246
6 · The paper itself

Abstract

The rational design of effective and safe non-viral gene vectors is largely dependent on the understanding of the structure-property relationship. We herein report the design of a new series of cationic, α-helical polypeptides with different side charged groups (amine and guanidine) and hydrophobicity, and mechanistically unraveled the effect of polypeptide structure on the gene delivery capability. Guanidine-containing polypeptides displayed superior membrane activities to their amine-containing analogues via the pore formation mechanism, and thus possessed notably higher transfection efficiencies. Elongating the hydrophobic side chain also potentiated the membrane activities of the polypeptides, while at the meantime caused higher cytotoxicities. Upon an optimal balance between membrane activity and cytotoxicity, maximal transfection efficiency was achieved which outperformed commercial reagent Lipofectamine™ 2000 (LPF2000) by 3-6 folds. This study thus provides mechanistic insights into the rational design of non-viral gene delivery vectors, and the best-performing materials identified also serve as a promising addition to the existing systems.

Indexed as

Gene Transfer TechniquesCationsHydrophobic and Hydrophilic InteractionsKineticsMagnetic Resonance SpectroscopyPeptidesCationsPeptidesGuanidineHydrophobicityNon-viral gene deliveryStructure–function relationshipα-Helical polypeptide

Identifiers

PMID24439403
PMCPMC4439012
OpenAlexW2113717063

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.