ArticleMicrobial cell factories2014
Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 130 citations in OpenAlex.
- Article
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- Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii.Biotechnology for biofuels and bioproducts · 2024Article
- Conversion of COProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Genome sequence of the entomopathogenic Serratia entomophila isolate 626 and characterisation of the species specific itaconate degradation pathway.BMC genomics · 2022Article
- Synergistic effects on itaconic acid production in engineered Aspergillus niger expressing the two distinct biosynthesis clusters from Aspergillus terreus and Ustilago maydis.Microbial cell factories · 2022Article
- Turning Inside Out: Filamentous Fungal Secretion and Its Applications in Biotechnology, Agriculture, and the Clinic.Journal of fungi (Basel, Switzerland) · 2021Review
- A Library ofFrontiers in bioengineering and biotechnology · 2021Article
- Consolidated bioprocessing of cellulose to itaconic acid by a co-culture of Trichoderma reesei and Ustilago maydis.Biotechnology for biofuels · 2020Article
- Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR-Cas9 system for succinic acid production from renewable biomass.Biotechnology for biofuels · 2020Article
- Metabolic engineering of an acid-tolerant yeast strainMetabolic engineering communications · 2020Article
- Metabolic engineering of an industrial3 Biotech · 2020Article
- Article
- Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers.Polymers · 2019Review
- Citric acid and itaconic acid accumulation: variations of the same story?Applied microbiology and biotechnology · 2019Review
- Identification of novel citramalate biosynthesis pathways inFungal biology and biotechnology · 2019Article
- Itaconic acid degradation inFungal biology and biotechnology · 2019Article
- Itaconic acid production in microorganisms.Biotechnology letters · 2018Review
- Article
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9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarboxylase, is missing. The introduction of the A. terreus cadA gene in A. niger exploits the high level of citric acid production (over 200 g per liter) and theoretically can lead to production levels of over 135 g per liter of itaconic acid in A. niger. Given the potential for higher production levels in A. niger, production of itaconic acid in this host was investigated.
resultsExpression of Aspergillus terreus cis-aconitate decarboxylase in Aspergillus niger resulted in the production of a low concentration (0.05 g/L) of itaconic acid. Overexpression of codon-optimized genes for cis-aconitate decarboxylase, a mitochondrial transporter and a plasma membrane transporter in an oxaloacetate hydrolase and glucose oxidase deficient A. niger strain led to highly increased yields and itaconic acid production titers. At these higher production titers, the effect of the mitochondrial and plasma membrane transporters was much more pronounced, with levels being 5-8 times higher than previously described.
conclusionsItaconic acid can be produced in A. niger by the introduction of the A. terreus cis-aconitate decarboxylase encoding cadA gene. This results in a low itaconic acid production level, which can be increased by codon-optimization of the cadA gene for A. niger. A second crucial requirement for efficient production of itaconic acid is the expression of the A. terreus mttA gene, encoding a putative mitochondrial transporter. Expression of this transporter results in a twenty-fold increase in the secretion of itaconic acid. Expression of the A. terreus itaconic acid cluster consisting of the cadA gene, the mttA gene and the mfsA gene results in A. niger strains that produce over twenty five-fold higher levels of itaconic acid and show a twenty-fold increase in yield compared to a strain expressing only CadA.
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