ArticleStem cells translational medicine2014
Amide-type local anesthetics and human mesenchymal stem cells: clinical implications for stem cell therapy.
Article in Stem cells translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Cartilage Regeneration in Human with Adipose Tissue-Derived Stem Cells: Current Status in Clinical Implications.BioMed research international · 2016Pooled it
- Cytoprotective Effects of Lipid Emulsion Against Bupivacaine-Induced Cytotoxicity in Human Rotator Cuff Fibroblasts.Antioxidants (Basel, Switzerland) · 2026Article
- Effects of local anesthetics on yield and differentiation of synovial mesenchymal stem cells.Scientific reports · 2026Article
- Intersection Between Local Anesthetics and Cancer Biology: What Now? Where Are We Going?Advanced biology · 2025Review
- Cytotoxic and antifungal effects of different teething oral gels: an in-vitro study.Head & face medicine · 2025Article
- An in vitro assessment of teething gels' effects on human gingival mesenchymal stem cells.BMC oral health · 2024Article
- Lidocaine intensifies the anti-osteogenic effect on inflammation-induced human dental pulp stem cells via mitogen-activated protein kinase inhibition.Journal of dental sciences · 2023Article
- Autologous adipose-derived stromal vascular fraction and platelet concentrates for the treatment of complex perianal fistulas.Techniques in coloproctology · 2023Article
- Lidocaine inhibits osteogenic differentiation of human dental pulp stem cellsThe Journal of international medical research · 2023Article
- Cytotoxicity of Local Anesthetics on Bone, Joint, and Muscle Tissues: A Narrative Review of the Current Literature.Journal of pain research · 2023Review
- Adipose-derived regenerative cells and lipotransfer in alleviating breast cancer-related lymphedema: An open-label phase I trial with 4 years of follow-up.Stem cells translational medicine · 2021Article
- A controlled release bupivacaine-alginate construct: Effect on chondrocyte hypertrophy conversion.Osteoarthritis and cartilage open · 2020Article
- Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cells.Stem cell research & therapy · 2020Article
- Viability and intracellular nitric oxide generation in the umbilical cord blood CD34Central-European journal of immunology · 2020Article
- Effect of Lidocaine on Viability and Gene Expression of Human Adipose-derived Mesenchymal Stem Cells: An in vitro Study.PM & R : the journal of injury, function, and rehabilitation · 2019Article
- Alginate encapsulation for bupivacaine delivery and mesenchymal stromal cell immunomodulatory cotherapy.Journal of inflammation research · 2019Article
- A Step-By-Step Surgical Protocol for the Treatment of Perianal Fistula with Adipose-Derived Mesenchymal Stem Cells.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2018Article
- Cytotoxicity of local anesthetics on rabbit adipose-derived mesenchymal stem cells during early chondrogenic differentiation.Experimental and therapeutic medicine · 2018Article
- Alginate-liposomal construct for bupivacaine delivery and MSC function regulation.Drug delivery and translational research · 2018Article
- The effect of local anesthetic on pro-inflammatory macrophage modulation by mesenchymal stromal cells.International immunopharmacology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the realm of regenerative medicine, human mesenchymal stem cells (hMSCs) are gaining attention as a cell source for the repair and regeneration of tissues spanning an array of medical disciplines. In orthopedics, hMSCs are often delivered in a site-specific manner at the area of interest and may require the concurrent application of local anesthetics (LAs). To address the implications of using hMSCs in combination with anesthetics for intra-articular applications, we investigated the effect that clinically relevant doses of amide-type LAs have on the viability of bone marrow-derived hMSCs and began to characterize the mechanism of LA-induced hMSC death. In our study, culture-expanded hMSCs from three donors were exposed to the amide-type LAs ropivacaine, lidocaine, bupivacaine, and mepivacaine. To replicate the physiological dilution of LAs once injected into the synovial capsule, each anesthetic was reduced to 12.5%, 25%, and 50% of the stock solution and incubated with each hMSC line for 40 minutes, 120 minutes, 360 minutes, and 24 hours. At each time point, cell viability assays were performed. We found that extended treatment with LAs for 24 hours had a significant impact on both hMSC viability and adhesion. In addition, hMSC treatment with three of the four anesthetics resulted in cell death via apoptosis following brief exposures. Ultimately, we concluded that amide-type LAs induce hMSC apoptosis in a time- and dose-dependent manner that may threaten clinical outcomes, following a similar trend that has been established between these particular anesthetics and articular chondrocytes both in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.