ArticleJournal of applied physiology (Bethesda, Md. : 1985)2014
Application of the [γ-32P] ATP kinase assay to study anabolic signaling in human skeletal muscle.
Article in Journal of applied physiology (Bethesda, Md. : 1985), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Acute effects of a ketone monoester, whey protein, or their coingestion on mTOR trafficking and protein-protein colocalization in human skeletal muscle.American journal of physiology. Cell physiology · 2024Trial
- High-intensity leg cycling alters the molecular response to resistance exercise in the arm muscles.Scientific reports · 2021Trial
- Skeletal muscle insulin signaling and whole-body glucose metabolism following acute sleep restriction in healthy males.Physiological reports · 2017Trial
- Sex differences in the effect of fish-oil supplementation on the adaptive response to resistance exercise training in older people: a randomized controlled trial.The American journal of clinical nutrition · 2017Trial
- Fish oil supplementation suppresses resistance exercise and feeding-induced increases in anabolic signaling without affecting myofibrillar protein synthesis in young men.Physiological reports · 2016Trial
- Molecular regulation of skeletal muscle mitochondrial biogenesis following blood flow-restricted aerobic exercise: a call to action.European journal of applied physiology · 2021Review
- Human skeletal muscle metabolic responses to 6 days of high-fat overfeeding are associated with dietary n-3PUFA content and muscle oxidative capacity.Physiological reports · 2020Article
- Leucine-enriched amino acids maintain peripheral mTOR-Rheb localization independent of myofibrillar protein synthesis and mTORC1 signaling postexercise.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Article
- Mechanical loading stimulates hypertrophy in tissue-engineered skeletal muscle: Molecular and phenotypic responses.Journal of cellular physiology · 2019Article
- Molecular regulation of human skeletal muscle protein synthesis in response to exercise and nutrients: a compass for overcoming age-related anabolic resistance.American journal of physiology. Cell physiology · 2019Review
- Obesity Alters the Muscle Protein Synthetic Response to Nutrition and Exercise.Frontiers in nutrition · 2019Review
- The Influence of Omega-3 Fatty Acids on Skeletal Muscle Protein Turnover in Health, Disuse, and Disease.Frontiers in nutrition · 2019Review
- Whole egg, but not egg white, ingestion induces mTOR colocalization with the lysosome after resistance exercise.American journal of physiology. Cell physiology · 2018Article
- Translocation and protein complex co-localization of mTOR is associated with postprandial myofibrillar protein synthesis at rest and after endurance exercise.Physiological reports · 2018Article
- Differential localization and anabolic responsiveness of mTOR complexes in human skeletal muscle in response to feeding and exercise.American journal of physiology. Cell physiology · 2017Article
- Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle.Scientific reports · 2017Article
- Skeletal muscle and resistance exercise training; the role of protein synthesis in recovery and remodeling.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Review
- The response of muscle protein synthesis following whole-body resistance exercise is greater following 40 g than 20 g of ingested whey protein.Physiological reports · 2016Article
- Fuel for the work required: a practical approach to amalgamating train-low paradigms for endurance athletes.Physiological reports · 2016Article
- Rapamycin does not prevent increases in myofibrillar or mitochondrial protein synthesis following endurance exercise.The Journal of physiology · 2015Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
Abstract
AMPK (AMP-dependant protein kinase)-mTORC1 (mechanistic target of rapamycin in complex 1)-p70S6K1 (ribosomal protein S6 kinase 1 of 70 kDa) signaling plays a crucial role in muscle protein synthesis (MPS). Understanding this pathway has been advanced by the application of the Western blot (WB) technique. However, because many components of the mTORC1 pathway undergo numerous, multisite posttranslational modifications, solely studying the phosphorylation changes of mTORC1 and its substrates may not adequately represent the true metabolic signaling processes. The aim of this study was to develop and apply a quantitative in vitro [γ-(32)P] ATP kinase assay (KA) for p70S6K1 to assess kinase activity in human skeletal muscle to resistance exercise (RE) and protein feeding. In an initial series of experiments the assay was validated in tissue culture and in p70S6K1-knockout tissues. Following these experiments, the methodology was applied to assess p70S6K1 signaling responses to a physiologically relevant stimulus. Six men performed unilateral RE followed by the consumption of 20 g of protein. Muscle biopsies were obtained at pre-RE, and 1 and 3 h post-RE. In response to RE and protein consumption, p70S6K1 activity as assessed by the KA was significantly increased from pre-RE at 1 and 3 h post-RE. However, phosphorylated p70S6K1(thr389) was not significantly elevated. AMPK activity was suppressed from pre-RE at 3 h post-RE, whereas phosphorylated ACC(ser79) was unchanged. Total protein kinase B activity also was unchanged after RE from pre-RE levels. Of the other markers we assessed by WB, 4EBP1(thr37/46) phosphorylation was the only significant responder, being elevated at 3 h post-RE from pre-RE. These data highlight the utility of the KA to study skeletal muscle plasticity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.