ArticleGut2014
Bacterial protein signals are associated with Crohn's disease.
Article in Gut, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 2 syntheses or guidelines pooled it, 92 citations in OpenAlex.
- Recent advances in characterizing the gastrointestinal microbiome in Crohn's disease: a systematic review.Inflammatory bowel diseases · 2015Pooled it
- Meta-analyses of human gut microbes associated with obesity and IBD.FEBS letters · 2014Pooled it
- Trial
- Short-chain fructo-oligosaccharides modulate gut microbiota composition and metabolism: dose-response assessment in anGut microbes reports · 2026Article
- A Knowledge-Guided Large Language Model Framework for Microbiome-Based Disease Diagnosis.Proceedings. IEEE International Conference on Bioinformatics and Biomedicine · 2025Article
- Metaproteomics reveals diet-induced changes in gut microbiome function according to Crohn's disease location.Microbiome · 2024Article
- Advances in multi-omics integrated analysis methods based on the gut microbiome and their applications.Frontiers in microbiology · 2024Review
- Glycoprotein 2 as a gut gate keeper for mucosal equilibrium between inflammation and immunity.Seminars in immunopathology · 2024Review
- Article
- T-independent responses to polysaccharides in humans mobilize marginal zone B cells prediversified against gut bacterial antigens.Science immunology · 2023Article
- Gut Microbiome Proteomics in Food Allergies.International journal of molecular sciences · 2023Review
- The role of gut microbiome in inflammatory bowel disease diagnosis and prognosis.United European gastroenterology journal · 2022Review
- Identification and characterization of murine glycoprotein 2-expressing intestinal dendritic cells.Scandinavian journal of immunology · 2022Article
- Article
- Novel Collaborative Weighted Non-negative Matrix Factorization Improves Prediction of Disease-Associated Human Microbes.Frontiers in microbiology · 2022Article
- Critical Assessment of MetaProteome Investigation (CAMPI): a multi-laboratory comparison of established workflows.Nature communications · 2021Article
- Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation.Microbiome · 2021Article
- Perturbed Microbiota/Immune Homeostasis in Multiple Sclerosis.Neurology(R) neuroimmunology & neuroinflammation · 2021Article
- Potential role of microbiome in Chronic Fatigue Syndrome/Myalgic Encephalomyelits (CFS/ME).Scientific reports · 2021Article
- High engraftment capacity of frozen ready-to-use human fecal microbiota transplants assessed in germ-free mice.Scientific reports · 2021Article
Corrections and comments
- Commented on by
Authors and funding
27 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveNo Crohn's disease (CD) molecular maker has advanced to clinical use, and independent lines of evidence support a central role of the gut microbial community in CD. Here we explore the feasibility of extracting bacterial protein signals relevant to CD, by interrogating myriads of intestinal bacterial proteomes from a small number of patients and healthy controls.
designWe first developed and validated a workflow-including extraction of microbial communities, two-dimensional difference gel electrophoresis (2D-DIGE), and LC-MS/MS-to discover protein signals from CD-associated gut microbial communities. Then we used selected reaction monitoring (SRM) to confirm a set of candidates. In parallel, we used 16S rRNA gene sequencing for an integrated analysis of gut ecosystem structure and functions.
resultsOur 2D-DIGE-based discovery approach revealed an imbalance of intestinal bacterial functions in CD. Many proteins, largely derived from Bacteroides species, were over-represented, while under-represented proteins were mostly from Firmicutes and some Prevotella members. Most overabundant proteins could be confirmed using SRM. They correspond to functions allowing opportunistic pathogens to colonise the mucus layers, breach the host barriers and invade the mucosae, which could still be aggravated by decreased host-derived pancreatic zymogen granule membrane protein GP2 in CD patients. Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins.
conclusionsThis study provides the first evidence that quantifiable bacterial protein signals are associated with CD, which can have a profound impact on future molecular diagnosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.