ArticleMolecular biology reports2014
uORF, a regulatory mechanism of the Arabidopsis polyamine oxidase 2.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Ribosome Pausing Negatively Regulates Protein Translation in Maize Seedlings during Dark-to-Light Transitions.International journal of molecular sciences · 2024Article
- What, where, and how: Regulation of translation and the translational landscape in plants.The Plant cell · 2024Review
- GUS Reporter-Aided Promoter Deletion Analysis ofInternational journal of molecular sciences · 2023Article
- Article
- Responses of Polyamine-Metabolic Genes to Polyamines and Plant Stress Hormones in Arabidopsis Seedlings.Cells · 2021Article
- Upstream Open Reading Frame Mediated Translation of WNK8 Is Required for ABA Response inInternational journal of molecular sciences · 2021Article
- Putrescine Depletion Affects Arabidopsis Root Meristem Size by Modulating Auxin and Cytokinin Signaling and ROS Accumulation.International journal of molecular sciences · 2021Article
- The tree of life of polyamine oxidases.Scientific reports · 2020Article
- uORFs: ImportantInternational journal of molecular sciences · 2020Review
- Metabolite Control of Translation by Conserved Peptide uORFs: The Ribosome as a Metabolite Multisensor.Plant physiology · 2020Article
- Genome-wide identification and characterization of the GDP-L-galactose phosphorylase gene family in bread wheat.BMC plant biology · 2019Article
- A uORF Represses the Transcription Factor AtHB1 in Aerial Tissues to Avoid a Deleterious Phenotype.Plant physiology · 2017Article
- Polyamine and Its Metabolite H2O2 Play a Key Role in the Conversion of Embryogenic Callus into Somatic Embryos in Upland Cotton (Gossypium hirsutum L.).Frontiers in plant science · 2015Article
- Physiological and molecular implications of plant polyamine metabolism during biotic interactions.Frontiers in plant science · 2014Review
- Recent advances in polyamine metabolism and abiotic stress tolerance.BioMed research international · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translational efficiency of an mRNA can be modulated by elements located in the 5'-untranslated region. The flavin-containing polyamine oxidases catabolize oxidative deamination of spermidine and spermine, thus contributing to polyamine homeostasis as well as diverse biological processes through their reaction products. In this study, we characterized the uORF of AtPAO2 gene using the GUS reporter gene. Transgenic lines harboring the native AtPAO2 promoter or the constitutive CaMV 35S promoter show that the uORF negatively affects GUS expression. Exogenous applications of PAs positively modulate GUS expression, thus alleviating the negative effect of AtPAO2 uORF, while treatments with MGBG inhibitor show an opposite effect. Our data suggest that AtPAO2 uORF regulatory mechanism is modulated by polyamines. In addition, we present a comparative in silico study of the uORFs identified in several plant transcripts encoding polyamine oxidases in both mono- and dicotyledonous plants as well as in the Bryophyte Physcomitrella patens. The polyamine oxidase uORF-encoded peptides are conserved among families and share conserved features such as their position, length, and amino acid sequence. Our findings provide new insights into the regulatory mechanism of polyamine oxidase genes and encourage further exploration to assess the biological significance of uORFs in the polyamine catabolic pathway.
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