ArticleMolecular therapy : the journal of the American Society of Gene Therapy2014
Strengthening the skin with topical delivery of keratinocyte growth factor-1 using a novel DNA plasmid.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Effect of Taspine hydrochloride on the repair of rat skin wounds by regulating keratinocyte growth factor signal.Bioengineered · 2022Article
- Glycosaminoglycans: Sweet as Sugar Targets for Topical Skin Anti-Aging.Clinical, cosmetic and investigational dermatology · 2021Review
- Stem Cells as a Target for the Delivery of Active Molecules to Skin by Topical Administration.International journal of molecular sciences · 2020Review
- Fibrin Nanoparticles Coupled with Keratinocyte Growth Factor Enhance the Dermal Wound-Healing Rate.ACS applied materials & interfaces · 2019Article
- Topical Reformulation of Valsartan for Treatment of Chronic Diabetic Wounds.The Journal of investigative dermatology · 2018Article
- Electrospun Fibers as a Dressing Material for Drug and Biological Agent Delivery in Wound Healing Applications.Bioengineering (Basel, Switzerland) · 2018Review
- Apratyramide, a Marine-Derived Peptidic Stimulator of VEGF-A and Other Growth Factors with Potential Application in Wound Healing.ACS chemical biology · 2018Article
- Endowing iPSC-Derived MSCs with Angiogenic and Keratinogenic Differentiation Potential: A Promising Cell Source for Skin Tissue Engineering.BioMed research international · 2018Article
- Raising Awareness Among Healthcare Providers about Epidermolysis Bullosa and Advancing Toward a Cure.The Journal of clinical and aesthetic dermatology · 2017Review
- Biochemical and Biophysical Cues in Matrix Design for Chronic and Diabetic Wound Treatment.Tissue engineering. Part B, Reviews · 2017Article
- Cellular GFP Toxicity and Immunogenicity: Potential Confounders in in Vivo Cell Tracking Experiments.Stem cell reviews and reports · 2016Review
- Dendritic epidermal T cells facilitate wound healing in diabetic mice.American journal of translational research · 2016Article
- Nucleic acid delivery into skin for the treatment of skin disease: Proofs-of-concept, potential impact, and remaining challenges.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
Abstract
Fragile skin, susceptible to decubitus ulcers and incidental trauma, is a problem particularly for the elderly and for those with spinal cord injury. Here, we present a simple approach to strengthen the skin by the topical delivery of keratinocyte growth factor-1 (KGF-1) DNA. In initial feasibility studies with the novel minimalized, antibiotic-free DNA expression vector, NTC8385-VA1, the reporter genes luciferase and enhanced green fluorescent protein were delivered. Transfection was documented when luciferase expression significantly increased after transfection. Microscopic imaging of enhanced green fluorescent protein-transfected skin showed green fluorescence in hair follicles, hair shafts, and dermal and superficial epithelial cells. With KGF-1 transfection, KGF-1 mRNA level and protein production were documented with quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry, respectively. Epithelial thickness of the transfected skin in the KGF group was significantly increased compared with the control vector group (26 ± 2 versus 16 ± 4 µm) at 48 hours (P = 0.045). Dermal thickness tended to be increased in the KGF group (255 ± 36 versus 162 ± 16 µm) at 120 hours (P = 0.057). Biomechanical assessment showed that the KGF-1-treated skin was significantly stronger than control vector-transfected skin. These findings indicate that topically delivered KGF-1 DNA plasmid can increase epithelial thickness and strength, demonstrating the potential of this approach to restore compromised skin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.