ArticleNature protocols2014
Imaging pericytes and capillary diameter in brain slices and isolated retinae.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 110 citations in OpenAlex.
- Rodent models and accompanying in vitro strategies for studying the etiology and therapy of Parkinson's disease.Journal of Parkinson's disease · 2026Review
- Kinetics of thromboxane A2 receptor-driven vascular tone in the cerebral cortex ex vivo.Microvascular research · 2025Article
- Pericyte pannexin1 controls cerebral capillary diameter and supports memory function.Nature communications · 2025Article
- The role of transient receptor potential melastatin channels in compressive force-induced contraction of primary cardiac pericytes.Physiological reports · 2025Article
- Exendin-4 improves cerebral ischemia by relaxing microvessels, rapidly increasing cerebral blood flow after reperfusion.Basic research in cardiology · 2025Article
- Response of capillaries and small arterioles to full-field flicker is not dependent on local ganglion cell thickness.Biomedical optics express · 2025Article
- Differential stiffness between brain vasculature and parenchyma promotes metastatic infiltration through vessel co-option.Nature cell biology · 2024Article
- Post-stroke hippocampal neurogenesis is impaired by microvascular dysfunction and PI3K signaling in cerebral amyloid angiopathy.Cell reports · 2024Article
- Establishment of an in vitro choroid complex system for vascular response screening.Scientific reports · 2024Article
- Distinct neurochemical influences on fMRI response polarity in the striatum.Nature communications · 2024Article
- Intermittent hypoxia protects against hypoxic-ischemic brain damage by inducing functional angiogenesis.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- SARS-CoV-2 triggers pericyte-mediated cerebral capillary constriction.Brain : a journal of neurology · 2023Article
- Assaying activity-dependent arteriole and capillary responses in brain slices.Neurophotonics · 2022Article
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- The Ca2+-gated channel TMEM16A amplifies capillary pericyte contraction and reduces cerebral blood flow after ischemia.The Journal of clinical investigation · 2022Article
- Article
- Hemodynamically Unloading the Distal Pulmonary Circulation in Pulmonary Hypertension: A Modeling Study.Journal of biomechanical engineering · 2022Article
- Pericyte Mapping in Cerebral Slices with the Far-red Fluorophore TO-PRO-3.Bio-protocol · 2021Article
- Article
- Combining whole-cell patch clamp and dye loading in acute brain slices with bulk RNA sequencing in embryonic to aged mice.STAR protocols · 2021Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The cerebral circulation is highly specialized, both structurally and functionally, and it provides a fine-tuned supply of oxygen and nutrients to active regions of the brain. Our understanding of blood flow regulation by cerebral arterioles has evolved rapidly. Recent work has opened new avenues in microvascular research; for example, it has been demonstrated that contractile pericytes found on capillary walls induce capillary diameter changes in response to neurotransmitters, suggesting that pericytes could have a role in neurovascular coupling. This concept is at odds with traditional models of brain blood flow regulation, which assume that only arterioles control cerebral blood flow. The investigation of mechanisms underlying neurovascular coupling at the capillary level requires a range of approaches, which involve unique technical challenges. Here we provide detailed protocols for the successful physiological and immunohistochemical study of pericytes and capillaries in brain slices and isolated retinae, allowing investigators to probe the role of capillaries in neurovascular coupling. This protocol can be completed within 6-8 h; however, immunohistochemical experiments may take 3-6 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.