ArticleInternational journal of molecular sciences2014
Purification and characterization of iso-ribonucleases from a novel thermophilic fungus.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 8 citations in OpenAlex.
- Clinical Evaluation of Microbial Communities and Associated Biofilms with Breast Augmentation Failure.Microorganisms · 2024Article
- Biofilms-Impacts on Human Health and Its Relevance to Space Travel.Microorganisms · 2020Review
- A Novel Cold-Adapted and Salt-Tolerant RNase R from Antarctic Sea-Ice BacteriumMolecules (Basel, Switzerland) · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A thermophilic fungus previously isolated from composted horse manure was found to produce extracellular iso-RNases that were purified 127.6-fold using a combination of size exclusion chromatography and a novel affinity membrane purification system. The extent of purification was determined electrophoretically using 4%-15% gradient polyacrylamide gels. RNase activity was dependent on the presence of a metal co-factor with significantly more activity with Zn2+ or Mn2+ than Mg2+. The RNases exhibited maximum activity at both pH 3.0 and pH 7.0 with no activity at pH 2.0 or 10.0. The optimal temperature for the iso-RNase was 70 °C. The molecular weight of the iso-RNase was determined to be 69 kDa using a Sephadex G-75 column.
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Registered trials
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