ArticleProceedings of the National Academy of Sciences of the United States of America2014
Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16α hydroxylase from Bupleurum falcatum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed, 201 citations in OpenAlex.
- Complete biosynthesis of the clinical agent escin Ia.Nature communications · 2026Article
- Exploring bacterial cytochrome P450s for selective activation of aliphatic C-H bonds in pentacyclic triterpenoids.Nature chemistry · 2026Article
- Genome-wide identification and expression analysis of the PnNPR-PnTGA gene family reveal its potential role in response to biotic stresses in Panax notoginseng.BMC plant biology · 2026Article
- Heterosis unveiled in root-related traits and saikosaponins content between triploid FFrontiers in plant science · 2026Article
- Comparative transcriptome analysis reveals abscisic acid-induced bHLH transcription factors involved in saikosaponin biosynthesis inPlant signaling & behavior · 2025Article
- Metabolic Engineering of Terpenoid Biosynthesis in Medicinal Plants: From Genomic Insights to Biotechnological Applications.Current issues in molecular biology · 2025Review
- Engineering Gypsophila elegans hairy root cultures to produce endosomal escape-enhancing saponins.Plant biotechnology journal · 2025Article
- A million shades of green: understanding and harnessing plant metabolic diversity.The EMBO journal · 2025Review
- Plant-derived triterpenoid saponins: multifaceted roles and bioengineering prospects.Plant cell reports · 2025Review
- Evolution of linear triterpenoid biosynthesis within the Euphorbia genus.Nature communications · 2025Article
- Integrated metabolomics and transcriptomics reveal differences in terpenoids and the molecular basis among the roots of three Bupleurum species.BMC plant biology · 2025Article
- Engineering Saccharomyces cerevisiae for medical applications.Microbial cell factories · 2025Review
- Exploration of In Situ Extraction for Enhanced Triterpenoid Production by Saccharomyces cerevisiae.Microbial biotechnology · 2024Article
- The biosynthesis of asiaticoside and madecassoside reveals tandem duplication-directed evolution of glycoside glycosyltransferases in the Apiales.Plant communications · 2024Article
- Isolation of C-29 oxygenated oleanane triterpenoids and a (+)-muurolene type sesquiterpenoid from the fruiting bodies of Fuscoporia torulosa and their bioactivities.Journal of natural medicines · 2024Article
- Advances in Engineering Nucleotide Sugar Metabolism for Natural Product Glycosylation inACS synthetic biology · 2024Review
- Functional Validation of the Cytochrome P450 FamilyBiomolecules · 2024Article
- RNA sequencing analysis revealsHorticulture research · 2024Article
- Plant In Vitro Culture Factories for Pentacyclic Triterpenoid Production.Advances in biochemical engineering/biotechnology · 2024Review
- Transcriptomic analysis reveals effects of fertilization towards growth and quality of Fritillariae thunbergii bulbus.PloS one · 2024Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The saikosaponins comprise oleanane- and ursane-type triterpene saponins that are abundantly present in the roots of the genus Bupleurum widely used in Asian traditional medicine. Here we identified a gene, designated CYP716Y1, encoding a cytochrome P450 monooxygenase from Bupleurum falcatum that catalyzes the C-16α hydroxylation of oleanane- and ursane-type triterpenes. Exploiting this hitherto unavailable enzymatic activity, we launched a combinatorial synthetic biology program in which we combined CYP716Y1 with oxidosqualene cyclase, P450, and glycosyltransferase genes available from other plant species and reconstituted the synthesis of monoglycosylated saponins in yeast. Additionally, we established a culturing strategy in which applying methylated β-cyclodextrin to the culture medium allows the sequestration of heterologous nonvolatile hydrophobic terpenes, such as triterpene sapogenins, from engineered yeast cells into the growth medium, thereby greatly enhancing productivity. Together, our findings provide a sound base for the development of a synthetic biology platform for the production of bioactive triterpene sapo(ge)nins.
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