Evidence map›Paper›PMID 24434086›Full record

ArticleThe Journal of molecular diagnostics : JMD2014

A novel subtyping assay for detection of Clostridium difficile virulence genes.

Stephanie L Angione, Aartik A Sarma, Aleksey Novikov, Leah Seward, Jennifer H Fieber, Leonard A Mermel, Anubhav Tripathi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of molecular diagnostics : JMD, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Stephanie L AngioneCenter for Biomedical Engineering, School of Engineering, Brown University, Providence, Rhode Island.
Aartik A SarmaCenter for Biomedical Engineering, School of Engineering, Brown University, Providence, Rhode Island.
Aleksey NovikovWarren Alpert Medical School, Brown University, Providence, Rhode Island.
Leah SewardDivision of Biology and Medicine, Brown University, Providence, Rhode Island.
Jennifer H FieberDivision of Biology and Medicine, Brown University, Providence, Rhode Island.
Leonard A MermelCenter for Biomedical Engineering, School of Engineering, Brown University, Providence, Rhode Island; Division of Infectious Diseases, Rhode Island Hospital, Providence, Rhode Island.
Anubhav TripathiCenter for Biomedical Engineering, School of Engineering, Brown University, Providence, Rhode Island. Electronic address: anubhav_tripathi@brown.edu.

Funding

PHS HHS 1R21 A1073808-01
6 · The paper itself

Abstract

This proof-of-concept study demonstrates the application of a novel nucleic acid detection platform to detect Clostridium difficile in subjects presenting with acute diarrheal symptoms. This method amplifies three genes associated with C. difficile infection, including genes and deletions (cdtB and tcdC) associated with hypervirulence attributed to the NAP1/027/BI strain. Amplification of DNA from the tcdB, tcdC, and cdtB genes was performed using a droplet-based sandwich platform with quantitative real-time PCR in microliter droplets to detect and identify the amplified fragments of DNA. The device and identification system are simple in design and can be integrated as a point-of-care test to help rapidly detect and identify C. difficile strains that pose significant health threats in hospitals and other health-care communities.

Indexed as

ADP Ribose TransferasesBacterial ProteinsBacterial ToxinsClostridioides difficileEnterocolitis, PseudomembranousHumansMultiplex Polymerase Chain ReactionPoint-of-Care SystemsReal-Time Polymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityVirulenceVirulence Factorsactin-specific ADP-ribosyltransferase, ClostridiumADP Ribose TransferasesBacterial ProteinsBacterial ToxinstoxB protein, Clostridium difficileVirulence Factors

Identifiers

PMID24434086
PMCPMC3937534

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.