ArticleVirology journal2014
The genome and proteome of Serratia bacteriophage η which forms unstable lysogens.
Article in Virology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Therapeutic and Diagnostic Potential of a Novel K1 Capsule Dependent Phage, JSSK01, and Its Depolymerase in Multidrug-ResistantInternational journal of molecular sciences · 2024Article
- Phage Annotation Guide: Guidelines for Assembly and High-Quality Annotation.PHAGE (New Rochelle, N.Y.) · 2021Article
- Characterization of NovelViruses · 2020Article
- Identification, Characterization, and Genomic Analysis of NovelInternational journal of molecular sciences · 2020Article
- Tackling Intrinsic Antibiotic Resistance in Serratia Marcescens with A Combination of Ampicillin/Sulbactam and Phage SALSA.Antibiotics (Basel, Switzerland) · 2020Article
- Characterization of CRISPR-Cas Systems inMicroorganisms · 2019Article
- Genomic, proteomic and bioinformatic analysis of two temperate phages in Roseobacter clade bacteria isolated from the deep-sea water.BMC genomics · 2017Article
- Bacteriophage therapy against Enterobacteriaceae.Virologica Sinica · 2015Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSerratia marcescens phage η is a temperate unclassified member of the Siphoviridae which had been reported as containing hypermodified guanine residues.
methodsThe DNA was characterized by enzymatic digestion followed by HPLC analysis of the nucleoside composition, and by DNA sequencing and proteomic analysis. Its ability to form stable lysogens and integrate was also investigated.
resultsEnzymatic digestion and HPLC analysis revealed phage η DNA did not contain modified bases. The genome sequence of this virus, determined using pyrosequencing, is 42,724 nucleotides in length with a mol% GC of 49.9 and is circularly permuted. Sixty-nine putative CDSs were identified of which 19 encode novel proteins. While seven close genetic relatives were identified, they shared sequence similarity with only genes 40 to 69 of the phage η genome, while gp1 to gp39 shared no conserved relationship. The structural proteome, determined by SDS-PAGE and mass spectrometry, revealed seven unique proteins. This phage forms very unstable lysogens with its host S. marcescens.
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