ArticlePeerJ2013
Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system.
Article in PeerJ, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Cyclic DNA remethylation following active demethylation at euchromatic regions in mouse embryonic stem cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Development of blastocyst complementation technology without contributions to gametes and the brain.Experimental animals · 2019Article
- Targeted genetic screening in mice through haploid embryonic stem cells identifies critical genes in bone development.PLoS biology · 2019Article
- Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection.Stem cell reports · 2018Article
- CRISPR/Cas9-mediated genome editing in naïve human embryonic stem cells.Scientific reports · 2017Article
- A versatile genetic tool: haploid cells.Stem cell research & therapy · 2017Review
- TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes.Scientific reports · 2017Article
- Generation of murine tumor cell lines deficient in MHC molecule surface expression using the CRISPR/Cas9 system.PloS one · 2017Article
- Regulation of CpG methylation by Dnmt and Tet in pluripotent stem cells.The Journal of reproduction and development · 2016Review
- CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice.Scientific reports · 2016Article
- Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems.Dong wu xue yan jiu = Zoological research · 2016Review
- Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection.BMC molecular biology · 2016Article
- Genome Editing Using Mammalian Haploid Cells.International journal of molecular sciences · 2015Review
- RNA-guided CRISPR-Cas technologies for genome-scale investigation of disease processes.Journal of hematology & oncology · 2015Review
- CrisprGE: a central hub of CRISPR/Cas-based genome editing.Database : the journal of biological databases and curation · 2015Article
- Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans.Scientific reports · 2014Article
- Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line.Genome research · 2014Article
- The new CRISPR-Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.Transgenic research · 2014Review
- Recent technological updates and clinical applications of induced pluripotent stem cells.The Korean journal of internal medicine · 2014Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haploid embryonic stem cells (ESCs) are useful for studying mammalian genes because disruption of only one allele can cause loss-of-function phenotypes. Here, we report the use of haploid ESCs and the CRISPR RNA-guided Cas9 nuclease gene-targeting system to manipulate mammalian genes. Co-transfection of haploid ESCs with vectors expressing Cas9 nuclease and single-guide RNAs (sgRNAs) targeting Tet1, Tet2, and Tet3 resulted in the complete disruption of all three genes and caused a loss-of-function phenotype with high efficiency (50%). Co-transfection of cells with vectors expressing Cas9 and sgRNAs targeting two loci on the same chromosome resulted in the creation of a large chromosomal deletion and a large inversion. Thus, the use of the CRISPR system in combination with haploid ESCs provides a powerful platform to manipulate the mammalian genome.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.