Evidence map›Paper›PMID 24431897›Full record

ArticleWorld journal of gastroenterology2013

Amelioration of carbon tetrachloride-induced cirrhosis and portal hypertension in rat using adenoviral gene transfer of Akt.

Gang Deng, Xiang-Jun Huang, Hong-Wu Luo, Fei-Zhou Huang, Xun-Yang Liu, Yong-Heng Wang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Gang DengGang Deng, Xiang-Jun Huang, Hong-Wu Luo, Fei-Zhou Huang, Xun-Yang Liu, Yong-Heng Wang, Department of Hepatobiliary and Pancreatic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China.
Xiang-Jun HuangGang Deng, Xiang-Jun Huang, Hong-Wu Luo, Fei-Zhou Huang, Xun-Yang Liu, Yong-Heng Wang, Department of Hepatobiliary and Pancreatic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China.
Hong-Wu LuoGang Deng, Xiang-Jun Huang, Hong-Wu Luo, Fei-Zhou Huang, Xun-Yang Liu, Yong-Heng Wang, Department of Hepatobiliary and Pancreatic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China.
Fei-Zhou HuangGang Deng, Xiang-Jun Huang, Hong-Wu Luo, Fei-Zhou Huang, Xun-Yang Liu, Yong-Heng Wang, Department of Hepatobiliary and Pancreatic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China.
Xun-Yang LiuGang Deng, Xiang-Jun Huang, Hong-Wu Luo, Fei-Zhou Huang, Xun-Yang Liu, Yong-Heng Wang, Department of Hepatobiliary and Pancreatic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China.
Yong-Heng WangGang Deng, Xiang-Jun Huang, Hong-Wu Luo, Fei-Zhou Huang, Xun-Yang Liu, Yong-Heng Wang, Department of Hepatobiliary and Pancreatic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China.
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate whether a virus constitutively expressing active Akt is useful to prevent cirrhosis induced by carbon tetrachloride (CCl4).

methodsUsing cre-loxp technique, we created an Ad-myr-HA-Akt virus, in which Akt is labeled by a HA tag and its expression is driven by myr promoter. Further, through measuring enzyme levels and histological structure, we determined the efficacy of this Ad-myr-HA-Akt virus in inhibiting the development of cirrhosis induced by CCl4 in rats. Lastly, using western blotting, we examined the expression levels and/or phosphorylation status of Akt, apoptotic mediators, endothelial nitric oxide synthase (eNOS), and markers for hepatic stellate cells activation to understand the underlying mechanisms of protective role of this virus.

resultsThe Ad-myr-HA-Akt virus was confirmed using polymerase chain reaction amplification of inserted Akt gene and sequencing for full length of inserted fragment, which was consistent with the sequence reported in the GenBank. The concentrations of Ad-myr-HA-Akt and adenoviral enhanced green fluorescent protein (Ad-EGFP) virus used in the current study were 5.5 × 10(11) vp/mL. The portal vein diameter, peak velocity of blood flow, portal blood flow and congestion index were significantly increased in untreated, saline and Ad-EGFP cirrhosis groups when compared to normal control after the virus was introduced to animal through tail veil injection. In contrast, these parameters in the Akt cirrhosis group were comparable to normal control group. Compared to the normal control, the liver function (Alanine aminotransferase, Aspartate aminotransferase and Albumin) was significantly impaired in the untreated, saline and Ad-EGFP cirrhosis groups. The Akt cirrhosis group showed significant improvement of liver function when compared to the untreated, saline and Ad-EGFP cirrhosis groups. The Hyp level and portal vein pressure in Akt cirrhosis groups were also significantly lower than other cirrhosis groups. The results of HE and Van Gieson staining indicated that Akt group has better preservation of histological structure and less fibrosis than other cirrhosis groups. The percentage of apoptotic cell was greatly less in Akt cirrhosis group than in other cirrhosis groups. Akt group showed positive HA tag and an increased level of phosphorylated Akt as well as decreased levels of Fas. In contrast, Caspase-3 and Caspase-9 levels in Akt group were significantly lower than other cirrhosis groups. Noticeable decrease of DR5 and α-SMA and increase of phosphorylated eNOS were observed in the Akt group when compared to other cirrhosis groups. The NO level in liver was significantly higher in Akt group than other cirrhosis groups, which was consistent with the level of phosphorylated eNOS in these groups.

conclusionThis study suggest that Ad-myr-HA-Akt virus is a useful tool to prevent CCl4-induced cirrhosis in rat model and Akt pathway may be a therapeutic target for human cirrhosis.

Indexed as

Carbon TetrachlorideGenetic VectorsGene Transfer TechniquesAdenoviridaeAnimalsApoptosisApoptosis Regulatory ProteinsDisease Models, AnimalEnzyme ActivationGenetic TherapyHepatic Stellate CellsHypertension, PortalLiver CirrhosisMaleNitric OxideNitric Oxide Synthase Type IIIApoptosis Regulatory ProteinsCarbon TetrachlorideNitric OxideNitric Oxide Synthase Type IIINos3 protein, ratProto-Oncogene Proteins c-aktAdenovirusAktApoptosisCarbon tetrachlorideCirrhosisGene transferRat

Identifiers

PMID24431897
PMCPMC3837279
OpenAlexW2009224674

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.