ArticleJournal of parasitic diseases : official organ of the Indian Society for Parasitology2013
ELISA and western blotting for the detection of Hsp70 and Hsp83 antigens of Leishmania donovani.
Article in Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 19 citations in OpenAlex.
- Fluorescence Immunosensor with Phage Antibodies for Heat Shock Protein 70 Detection.Biosensors · 2026Article
- Proteome identification of common immunological proteins of two nematode parasites.Parasites, hosts and diseases · 2024Article
- An immunoproteomics approach to identifyParasitology · 2020Article
- Leishmaniasis diagnosis: an update on the use of parasitological, immunological and molecular methods.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Review
- Early antibody response and clinical outcome in experimental canine leishmaniasis.Scientific reports · 2019Article
- Immunoproteomic profile of Trichinella spiralis adult worm proteins recognized by early infection sera.Parasites & vectors · 2015Article
- Epitope mapping of the HSP83.1 protein of Leishmania braziliensis discloses novel targets for immunodiagnosis of tegumentary and visceral clinical forms of leishmaniasis.Clinical and vaccine immunology : CVI · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
Visceral leishmaniasis (VL) is endemic in the tropical, sub-tropical regions of Asia, Africa, the Mediterranean, Southern Europe and South and Central America. Approximately 500,000 new cases are reported annually. Classically the diagnosis of VL is confirmed by the demonstration of the parasite in aspirates of spleen, bone marrow or liver which can yield false negative results, also these methods are invasive. In this study, we aimed to evaluate the serodiagnostic potential of two heat shock proteins (Hsps) i.e. Hsp70 and Hsp83 in combination by ELISA and western blotting in mouse model. Parasite proteins were separated by SDS-PAGE and transferred to PVDF membranes which were further incubated with serum samples of infected mice for visualizing different bands. Both the bands i.e. Hsp70 and Hsp83 were simultaneously visualized in all infected groups on different post infection days. The presence of both the antigens was also detected by sandwich ELISA. The results suggest that the simultaneous occurrence of both the antigens Hsp70 and Hsp83 may be useful in serodiagnostic assay of VL as it may reduce the need for traumatic bone marrow sampling and risky spleen aspiration.
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