ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2013
Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Resistance Evaluation for Native Potato Accessions against Late Blight Disease and Potato Cyst Nematodes by Molecular Markers and Phenotypic Screening in India.Life (Basel, Switzerland) · 2022Article
- Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants ofPlants (Basel, Switzerland) · 2022Article
- In vitro Regeneration of Hyssopus officinalis L. and Plant Genetic Similarity.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2021Article
- Random Amplified Polymorphic DNA (RAPD) and Derived Techniques.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Amplified Fragment Length Polymorphism: Applications and Recent Developments.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Dynamic Changes of DNA Methylation During Wild Strawberry (Frontiers in plant science · 2021Article
- Auxins in potato: molecular aspects and emerging roles in tuber formation and stress resistance.Plant cell reports · 2019Review
- Thidiazuron (TDZ) induced organogenesis and clonal fidelity studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Review
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6 authors.
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Abstract
The genetic stability of in vitro propagated potato microtubers was assessed using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Microtubers were developed through in vitro from potato microplants using standardized protocols. The microtubers were conserved for 1 year under three different culture media and consequently microplants were regenerated for the DNA analyses. During the study, a total of 38 (10 RAPD, 11 ISSR, 12 SSR and 5 AFLP) primers produced a total of 407 (58 RAPD, 56 ISSR, 96 SSR and 197 AFLP) clear, distinct and reproducible amplicons. Cluster analysis revealed 100 % genetic similarity among the mother plant and its derivatives within the clusters by SSR, ISSR and RAPD analyses, whereas AFLP analysis revealed from 85 to 100 % genetic similarity. Dendrogram analysis based on the Jaccard's coefficient classified the genotypes into five clusters (I-V), each cluster consisting of mother plant and its derivatives. Principal component analysis (PCA) also plotted mother plant and its genotypes of each cluster together. Based on our results, it is concluded that AFLP is the best method followed by SSR, ISSR and RAPD to detect genetic stability of in vitro conserved potato microtubers. The in vitro conservation medium (T2) is a safe method for conservation of potato microtubers to produce true-to-type plans.
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