ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2013
Transgenics in groundnut (Arachis hypogaea L.) expressing cry1AcF gene for resistance to Spodoptera litura (F.).
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Advances in Tissue Culture-Free Genetic Engineering and Genome Editing of Peanut.Molecular biotechnology · 2026Review
- Next-Generation Precision Breeding in Peanut (Insects · 2026Review
- CRISPR-Mediated Genome Editing in Peanuts: Unlocking Trait Improvement for a Sustainable Future.Plants (Basel, Switzerland) · 2025Review
- Progress in genetic engineering and genome editing of peanuts: revealing the future of crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- Advances in omics research on peanut response to biotic stresses.Frontiers in plant science · 2023Review
- Amenability of anGM crops & food · 2022Article
- Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen GeneInternational journal of molecular sciences · 2022Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Imaging flowers: a guide to current microscopy and tomography techniques to study flower development.Journal of experimental botany · 2020Review
- Transgenic Cotton (Frontiers in plant science · 2020Article
- Article
- Transgenic approaches for genetic improvement in groundnut (Journal, genetic engineering & biotechnology · 2018Review
- Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in crops.BMC biotechnology · 2017Article
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Authors and funding
7 authors.
Funding
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Abstract
Large number of primary transgenic events were generated in groundnut by an Agrobacterium mediated, in planta transformation method to assess the efficacy of cry1AcF against the Spodoptera litura. The amplification of required size fragment of 750 bp with npt II primers and 901 bp with cry1AcF gene primers confirmed the integration of the gene. The expression of the cry gene was ascertained by ELISA in T2 generation, and the maximum concentration of cry protein in transgenic plants reached approximately 0.82 μg/g FW. Further, Southern blot analysis of ten T2 transgenic plants proved that transgene had been integrated in the genome of all the plants and Northern analysis of the same plants demonstrated the active expression of cry1AcF gene. The highest mean % larval mortalities 80.0 and 85.0 with an average mean % larval mortalities 16.25 (n = 369) and 26.0 (n = 80) were recorded in T1 and T2 generations, respectively. Segregation analysis of the selected lines in the T3 generation demonstrated homozygous nature. This clearly proved that though there is considerable improvement in average mean % larval mortality in T2 generation, the cry1AcF gene was effective against S. litura only to some extent.
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