ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Investigation of synapse formation and function in a glutamatergic-GABAergic two-neuron microcircuit.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- An integratediScience · 2026Article
- Decoding chronic pain: the glutamate-GABA tug of war in the cerebral cortex.Frontiers in molecular neuroscience · 2025Review
- GABAergic synaptic components are largely preserved across human and mouse neuronal models.Frontiers in cellular neuroscience · 2025Article
- Utilising activity patterns of a complex biophysical network model to optimise intra-striatal deep brain stimulation.Scientific reports · 2024Article
- Understanding the mechanism of action and clinical effects of neuroactive steroids and GABAergic compounds in major depressive disorder.Translational psychiatry · 2023Review
- EHMT1 regulates Parvalbumin-positive interneuron development and GABAergic input in sensory cortical areas.Brain structure & function · 2020Article
- Autaptic Cultures: Methods and Applications.Frontiers in synaptic neuroscience · 2020Article
- Glutamatergic Innervation onto Striatal Neurons Potentiates GABAergic Synaptic Output.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.Scientific reports · 2019Article
- Glutamatergic and dopaminergic modulation of cortico-striatal circuits probed by dynamic calcium imaging of networks reconstructed in microfluidic chips.Scientific reports · 2018Article
- Differential pH Dynamics in Synaptic Vesicles From Intact Glutamatergic and GABAergic Synapses.Frontiers in synaptic neuroscience · 2018Article
- The antipsychotic drugs olanzapine and haloperidol modify network connectivity and spontaneous activity of neural networks in vitro.Scientific reports · 2017Article
- PTEN Loss Increases the Connectivity of Fast Synaptic Motifs and Functional Connectivity in a Developing Hippocampal Network.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Distinct Localization of SNAP47 Protein in GABAergic and Glutamatergic Neurons in the Mouse and the Rat Hippocampus.Frontiers in neuroanatomy · 2017Article
- Live-Cell, Label-Free Identification of GABAergic and Non-GABAergic Neurons in Primary Cortical Cultures Using Micropatterned Surface.PloS one · 2016Article
- Remodeling and Tenacity of Inhibitory Synapses: Relationships with Network Activity and Neighboring Excitatory Synapses.PLoS computational biology · 2015Article
- Molecular mechanisms governing Ca(2+) regulation of evoked and spontaneous release.Nature neuroscience · 2015Review
- Shank1 regulates excitatory synaptic transmission in mouse hippocampal parvalbumin-expressing inhibitory interneurons.The European journal of neuroscience · 2015Article
- Vesicular glutamate transporter expression level affects synaptic vesicle release probability at hippocampal synapses in culture.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Loss of mTOR repressors Tsc1 or Pten has divergent effects on excitatory and inhibitory synaptic transmission in single hippocampal neuron cultures.Frontiers in molecular neuroscience · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural circuits are composed of mainly glutamatergic and GABAergic neurons, which communicate through synaptic connections. Many factors instruct the formation and function of these synapses; however, it is difficult to dissect the contribution of intrinsic cell programs from that of extrinsic environmental effects in an intact network. Here, we perform paired recordings from two-neuron microculture preparations of mouse hippocampal glutamatergic and GABAergic neurons to investigate how synaptic input and output of these two principal cells develop. In our reduced preparation, we found that glutamatergic neurons showed no change in synaptic output or input regardless of partner neuron cell type or neuronal activity level. In contrast, we found that glutamatergic input caused the GABAergic neuron to modify its output by way of an increase in synapse formation and a decrease in synaptic release efficiency. These findings are consistent with aspects of GABAergic synapse maturation observed in many brain regions. In addition, changes in GABAergic output are cell wide and not target-cell specific. We also found that glutamatergic neuronal activity determined the AMPA receptor properties of synapses on the partner GABAergic neuron. All modifications of GABAergic input and output required activity of the glutamatergic neuron. Because our system has reduced extrinsic factors, the changes we saw in the GABAergic neuron due to glutamatergic input may reflect initiation of maturation programs that underlie the formation and function of in vivo neural circuits.
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