ArticleScience translational medicine2014
Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates.
Article in Science translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
78 citing papers in PubMed.
- Multiplexed video hyperspectral microendoscopy reveals tumor-immune dynamics in vivo.Research square · 2026Article
- Single Cell RNA Sequencing in Colorectal Cancer Immunology: Recent Updates, Application, and Emerging Challenges.Current molecular medicine · 2026Review
- Mass spectrometry-based proteomics of FFPE tissues: progress, limitations, and clinical translation barriers.Clinical proteomics · 2025Review
- Advances and challenges in precision imaging.The Lancet. Oncology · 2025Review
- Minimally invasive biopsy-based diagnostics in support of precision cancer medicine.Molecular oncology · 2024Review
- Detection of >400 Cluster of Differentiation Biomarkers and Pathway Proteins in Single Immune Cells by Cyclic MultiplexAnalytical chemistry · 2024Article
- Ultra high content analyses of circulating and tumor associated hybrid cells reveal phenotypic heterogeneity.Scientific reports · 2024Article
- Recent methodological advances towards single-cell proteomics.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2023Review
- Shooting for multiplexed pathology with Orion.Nature cancer · 2023Article
- Light-Deactivated Fluorescent Probes (FLASH-Off) for Multiplexed Imaging.Journal of the American Chemical Society · 2023Article
- Article
- Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes.Nature biotechnology · 2022Article
- Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles.ACS nano · 2022Review
- Multiplexed single-cell analysis of FNA allows accurate diagnosis of salivary gland tumors.Cancer cytopathology · 2022Review
- Integrated Analytical System for Clinical Single-Cell Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Cyclic microchip assay for measurement of hundreds of functional proteins in single neurons.Nature communications · 2022Article
- Oligonucleotide conjugated antibody strategies for cyclic immunostaining.Scientific reports · 2021Article
- TRIPODD: a Novel Fluorescence Imaging Platform for In Situ Quantification of Drug Distribution and Therapeutic Response.Molecular imaging and biology · 2021Article
- Multiplex protein analysis for the study of glaucoma.Expert review of proteomics · 2021Review
- Single-cell proteomic analysis.WIREs mechanisms of disease · 2021Review
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Immunohistochemistry-based clinical diagnoses require invasive core biopsies and use a limited number of protein stains to identify and classify cancers. We introduce a technology that allows analysis of hundreds of proteins from minimally invasive fine-needle aspirates (FNAs), which contain much smaller numbers of cells than core biopsies. The method capitalizes on DNA-barcoded antibody sensing, where barcodes can be photocleaved and digitally detected without any amplification steps. After extensive benchmarking in cell lines, this method showed high reproducibility and achieved single-cell sensitivity. We used this approach to profile ~90 proteins in cells from FNAs and subsequently map patient heterogeneity at the protein level. Additionally, we demonstrate how the method could be used as a clinical tool to identify pathway responses to molecularly targeted drugs and to predict drug response in patient samples. This technique combines specificity with ease of use to offer a new tool for understanding human cancers and designing future clinical trials.
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Registered trials
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