ArticleMolecular biology of the cell2014
An H3K9/S10 methyl-phospho switch modulates Polycomb and Pol II binding at repressed genes during differentiation.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
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- A systemic cell cycle block impacts stage-specific histone modification profiles during Xenopus embryogenesis.PLoS biology · 2021Article
- Large-scale identification of protein histidine methylation in human cells.NAR genomics and bioinformatics · 2021Article
- Methylation multiplicity and its clinical values in cancer.Expert reviews in molecular medicine · 2021Review
- One-Pot Quantitative Top- and Middle-Down Analysis of GluC-Digested Histone H4.Journal of the American Society for Mass Spectrometry · 2019Article
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- The meiotic phosphatase GSP-2/PP1 promotes germline immortality and small RNA-mediated genome silencing.PLoS genetics · 2019Article
- Coordination between TGF-β cellular signaling and epigenetic regulation during epithelial to mesenchymal transition.Epigenetics & chromatin · 2019Article
- N-terminal acetylation and methylation differentially affect the function of MYL9.The Biochemical journal · 2018Article
- The methyltransferase NSD3 promotes antiviral innate immunity via direct lysine methylation of IRF3.The Journal of experimental medicine · 2017Article
- Signaling coupled epigenomic regulation of gene expression.Oncogene · 2017Review
- Histone H4 Methyltransferase Suv420h2 Maintains Fidelity of Osteoblast Differentiation.Journal of cellular biochemistry · 2017Article
- Radiation Resistance in KRAS-Mutated Lung Cancer Is Enabled by Stem-like Properties Mediated by an Osteopontin-EGFR Pathway.Cancer research · 2017Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Methylated histones H3K9 and H3K27 are canonical epigenetic silencing modifications in metazoan organisms, but the relationship between the two modifications has not been well characterized. H3K9me3 coexists with H3K27me3 in pluripotent and differentiated cells. However, we find that the functioning of H3K9me3 is altered by H3S10 phosphorylation in differentiated postmitotic osteoblasts and cycling B cells. Deposition of H3K9me3/S10ph at silent genes is partially mediated by the mitogen- and stress-activated kinases (MSK1/2) and the Aurora B kinase. Acquisition of H3K9me3/S10ph during differentiation correlates with loss of paused S5 phosphorylated RNA polymerase II, which is present on Polycomb-regulated genes in embryonic stem cells. Reduction of the levels of H3K9me3/S10ph by kinase inhibition results in increased binding of RNAPIIS5ph and the H3K27 methyltransferase Ezh1 at silent promoters. Our results provide evidence of a novel developmentally regulated methyl-phospho switch that modulates Polycomb regulation in differentiated cells and stabilizes repressed states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.