ArticleJournal of visualized experiments : JoVE2013
Laser-scanning photostimulation of optogenetically targeted forebrain circuits.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 6 citations in OpenAlex.
- Expansion of human hepatocytes and their application in three-dimensional culture and genetic manipulation.Nature protocols · 2025Article
- Mechanism of thermodormancy induction in Fraxinus mandshurica seeds is related to changes in the endosperm.Planta · 2025Article
- Functional Topography and Development of Inhibitory Reticulothalamic Barreloid Projections.Frontiers in neuroanatomy · 2018Article
- Commissural functional topography of the inferior colliculus assessed in vitro.Hearing research · 2015Article
- Two types of auditory glutamatergic synapses and their implications for repairing damaged central auditory pathways.Neural regeneration research · 2014Article
- Frequency transformation in the auditory lemniscal thalamocortical system.Frontiers in neural circuits · 2014Review
- Inhibition of mammillary body neurons by direct activation of Group II metabotropic glutamate receptors.Neurotransmitter (Houston, Tex.)Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The sensory forebrain is composed of intricately connected cell types, of which functional properties have yet to be fully elucidated. Understanding the interactions of these forebrain circuits has been aided recently by the development of optogenetic methods for light-mediated modulation of neuronal activity. Here, we describe a protocol for examining the functional organization of forebrain circuits in vitro using laser-scanning photostimulation of channelrhodopsin, expressed optogenetically via viral-mediated transfection. This approach also exploits the utility of cre-lox recombination in transgenic mice to target expression in specific neuronal cell types. Following transfection, neurons are physiologically recorded in slice preparations using whole-cell patch clamp to measure their evoked responses to laser-scanning photostimulation of channelrhodopsin expressing fibers. This approach enables an assessment of functional topography and synaptic properties. Morphological correlates can be obtained by imaging the neuroanatomical expression of channelrhodopsin expressing fibers using confocal microscopy of the live slice or post-fixed tissue. These methods enable functional investigations of forebrain circuits that expand upon more conventional approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.