ArticleJournal of visualized experiments : JoVE2013
Intravital video microscopy measurements of retinal blood flow in mice.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Loss of TRPV2-mediated blood flow autoregulation recapitulates diabetic retinopathy in rats.JCI insight · 2022Article
- Mural Cell SRF Controls Pericyte Migration, Vessel Patterning and Blood Flow.Circulation research · 2022Article
- In Vivo Capillary Structure and Blood Cell Flux in the Normal and Diabetic Mouse Eye.Investigative ophthalmology & visual science · 2022Article
- Intravital Imaging of Circulating Red Blood Cells in the Retinal Vasculature of Growing Mice.Translational vision science & technology · 2021Article
- Understanding the in vivo Fate of Advanced Materials by Imaging.Advanced functional materials · 2020Article
- Altered Retinal Hemodynamics and Mean Circulation Time in Spontaneously Hypertensive Rats.Investigative ophthalmology & visual science · 2020Article
- Retinal Physiology and Circulation: Effect of Diabetes.Comprehensive Physiology · 2020Review
- Article
- Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis.Journal of ophthalmology · 2019Article
- Deep insights: intravital imaging with two-photon microscopy.Pflugers Archiv : European journal of physiology · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Alterations in retinal blood flow can contribute to, or be a consequence of, ocular disease and visual dysfunction. Therefore, quantitation of altered perfusion can aid research into the mechanisms of retinal pathologies. Intravital video microscopy of fluorescent tracers can be used to measure vascular diameters and bloodstream velocities of the retinal vasculature, specifically the arterioles branching from the central retinal artery and of the venules leading into the central retinal vein. Blood flow rates can be calculated from the diameters and velocities, with the summation of arteriolar flow, and separately venular flow, providing values of total retinal blood flow. This paper and associated video describe the methods for applying this technique to mice, which includes 1) the preparation of the eye for intravital microscopy of the anesthetized animal, 2) the intravenous infusion of fluorescent microspheres to measure bloodstream velocity, 3) the intravenous infusion of a high molecular weight fluorescent dextran, to aid the microscopic visualization of the retinal microvasculature, 4) the use of a digital microscope camera to obtain videos of the perfused retina, and 5) the use of image processing software to analyze the video. The same techniques can be used for measuring retinal blood flow rates in rats.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.