ArticleJournal of visualized experiments : JoVE2013
In situ TEM of biological assemblies in liquid.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 8 citations in OpenAlex.
- Tutorial on In Situ andACS nano · 2024Review
- Facile hermetic TEM grid preparation for molecular imaging of hydrated biological samples at room temperature.Nature communications · 2023Article
- Facile hermetic TEM grid preparation for molecular imaging of hydrated biological samples at room temperature.Research square · 2023Article
- Exobiology of the Venusian Clouds: New Insights into Habitability through Terrestrial Models and Methods of Detection.Astrobiology · 2021Article
- Liquid electron microscopy: then, now and future.Applied microscopy · 2019Review
- Liquid-Cell Electron Tomography of Biological Systems.Nano letters · 2019Article
- Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells.Viruses · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Researchers regularly use Transmission Electron Microscopes (TEMs) to examine biological entities and to assess new materials. Here, we describe an additional application for these instruments- viewing viral assemblies in a liquid environment. This exciting and novel method of visualizing biological structures utilizes a recently developed microfluidic-based specimen holder. Our video article demonstrates how to assemble and use a microfluidic holder to image liquid specimens within a TEM. In particular, we use simian rotavirus double-layered particles (DLPs) as our model system. We also describe steps to coat the surface of the liquid chamber with affinity biofilms that tether DLPs to the viewing window. This permits us to image assemblies in a manner that is suitable for 3D structure determination. Thus, we present a first glimpse of subviral particles in a native liquid environment.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.