ArticleThe Journal of biological chemistry2014
Glycerol dehydrogenase plays a dual role in glycerol metabolism and 2,3-butanediol formation in Klebsiella pneumoniae.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 78 citations in OpenAlex.
- A New Family of Electron-Bifurcating Enzymes Represented by Ferredoxin- and NAD-Dependent Glycerol-3-Phosphate Oxidoreductase.Biochemistry · 2026Article
- Knockout of glycerol metabolic pathways enables efficient mycolicibacterial phytosterol conversion using glycerol as cosovlent.Applied microbiology and biotechnology · 2025Article
- High production of enantiopure (R,R)-2,3-butanediol from crude glycerol by Klebsiella pneumoniae with an engineered oxidative pathway and a two-stage agitation strategy.Microbial cell factories · 2024Article
- Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics.Microorganisms · 2023Article
- Production ofSynthetic and systems biotechnology · 2023Article
- Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production.Microbial cell factories · 2021Article
- Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae.Biotechnology for biofuels · 2021Article
- Biochemical and Molecular Characterization of Glycerol Dehydrogenas fromJournal of microbiology and biotechnology · 2020Article
- Adaptability of Klebsiella pneumoniae 2e, a Newly Isolated 1,3-Propanediol-Producing Strain, to Crude Glycerol as Revealed by Genomic Profiling.Applied and environmental microbiology · 2019Article
- Structure of glycerol dehydrogenase (GldA) from Escherichia coli.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress.Scientific reports · 2017Article
- The role of budABC on 1,3-propanediol and 2,3-butanediol production from glycerol in Klebsiella pneumoniae CICIM B0057.Bioengineered · 2016Article
- High Production of 3-Hydroxypropionic Acid in Klebsiella pneumoniae by Systematic Optimization of Glycerol Metabolism.Scientific reports · 2016Article
- Mechanism of 2,3-butanediol stereoisomers formation in a newly isolated Serratia sp. T241.Scientific reports · 2016Article
- Engineering Bacillus licheniformis for the production of meso-2,3-butanediol.Biotechnology for biofuels · 2016Article
- Systems-Wide Prediction of Enzyme Promiscuity Reveals a New Underground Alternative Route for Pyridoxal 5'-Phosphate Production in E. coli.PLoS computational biology · 2016Article
- Cryoprotectant Toxicity: Facts, Issues, and Questions.Rejuvenation research · 2015Review
- Enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in Bacillus amyloliquefaciens.Microbial cell factories · 2015Article
- R-acetoin accumulation and dissimilation in Klebsiella pneumoniae.Journal of industrial microbiology & biotechnology · 2015Article
- Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production.Journal of industrial microbiology & biotechnology · 2015Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Glycerol dehydrogenase (GDH) is an important polyol dehydrogenase for glycerol metabolism in diverse microorganisms and for value-added utilization of glycerol in the industry. Two GDHs from Klebsiella pneumoniae, DhaD and GldA, were expressed in Escherichia coli, purified and characterized for substrate specificity and kinetic parameters. Both DhaD and GldA could catalyze the interconversion of (3R)-acetoin/(2R,3R)-2,3-butanediol or (3S)-acetoin/meso-2,3-butanediol, in addition to glycerol oxidation. Although purified GldA appeared more active than DhaD, in vivo inactivation and quantitation of their respective mRNAs indicate that dhaD is highly induced by glycerol and plays a dual role in glycerol metabolism and 2,3-butanediol formation. Complementation in K. pneumoniae further confirmed the dual role of DhaD. Promiscuity of DhaD may have vital physiological consequences for K. pneumoniae growing on glycerol, which include balancing the intracellular NADH/NAD(+) ratio, preventing acidification, and storing carbon and energy. According to the kinetic response of DhaD to modified NADH concentrations, DhaD appears to show positive homotropic interaction with NADH, suggesting that the physiological role could be regulated by intracellular NADH levels. The co-existence of two functional GDH enzymes might be due to a gene duplication event. We propose that whereas DhaD is specialized for glycerol utilization, GldA plays a role in backup compensation and can turn into a more proficient catalyst to promote a survival advantage to the organism. Revelation of the dual role of DhaD could further the understanding of mechanisms responsible for enzyme evolution through promiscuity, and guide metabolic engineering methods of glycerol metabolism.
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