ArticleJournal of the American Chemical Society2014
Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Voltage imaging: A practical guide.Neuron · 2026Review
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.Science advances · 2025Article
- Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators.bioRxiv : the preprint server for biology · 2024Article
- Current Status and Future Strategies for Advancing Functional Circuit MappingThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Article
- Diminishing neuronal acidification by channelrhodopsins with low proton conduction.bioRxiv : the preprint server for biology · 2023Article
- Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.Science advances · 2021Article
- Imaging Voltage with Microbial Rhodopsins.Frontiers in molecular biosciences · 2021Review
- Photocycle Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1.International journal of molecular sciences · 2019Article
- Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor QuasAr1.International journal of molecular sciences · 2019Article
- All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human iPSC-Derived Motor Neuron Model of ALS.Stem cell reports · 2018Article
- Voltage imaging with genetically encoded indicators.Current opinion in chemical biology · 2017Review
- Roadmap on neurophotonics.Journal of optics (2010) · 2016Article
- Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors.Current opinion in biotechnology · 2016Review
- Design and Synthesis of a Calcium-Sensitive Photocage.Angewandte Chemie (International ed. in English) · 2016Article
- Two-Photon Lifetime Imaging of Voltage Indicating Proteins as a Probe of Absolute Membrane Voltage.Biophysical journal · 2015Article
- The evolving capabilities of rhodopsin-based genetically encoded voltage indicators.Current opinion in chemical biology · 2015Review
- Initial photophysical characterization of the proteorhodopsin optical proton sensor (PROPS).Frontiers in neuroscience · 2015Article
- Imaging GFP-based reporters in neurons with multiwavelength optogenetic control.Biophysical journal · 2014Article
- One gene, many neuropsychiatric disorders: lessons from Mendelian diseases.Nature neuroscience · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We developed a technique, "flash memory", to record a photochemical imprint of the activity state--firing or not firing--of a neuron at a user-selected moment in time. The key element is an engineered microbial rhodopsin protein with three states. Two nonfluorescent states, D1 and D2, exist in a voltage-dependent equilibrium. A stable fluorescent state, F, is reached by a photochemical conversion from D2. When exposed to light of a wavelength λ(write), population transfers from D2 to F, at a rate determined by the D1 ⇌ D2 equilibrium. The population of F maintains a record of membrane voltage which persists in the dark. Illumination at a later time at a wavelength λ(read) excites fluorescence of F, probing this record. An optional third flash at a wavelength λ(reset) converts F back to D2, for a subsequent write-read cycle. The flash memory method offers the promise to decouple the recording of neural activity from its readout. In principle, the technique may enable one to generate snapshots of neural activity in a large volume of neural tissue, e.g., a complete mouse brain, by circumventing the challenge of imaging a large volume with simultaneous high spatial and high temporal resolution. The proof-of-principle flash memory sensors presented here will need improvements in sensitivity, speed, brightness, and membrane trafficking before this goal can be realized.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.