ArticlePloS one2014
Dynamic association with donor cell filopodia and lipid-modification are essential features of Wnt8a during patterning of the zebrafish neuroectoderm.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 63 citations in OpenAlex.
- Membrane lipid composition and rapid endocytic retrieval modulate Wingless secretion.The EMBO journal · 2026Article
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- Dynamics of BMP signaling and stable gene expression in the early Drosophila embryo.Biology open · 2024Article
- Soluble Frizzled-related proteins promote exosome-mediated Wnt re-secretion.Communications biology · 2024Article
- Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation.Development (Cambridge, England) · 2023Article
- Extracellular Vesicles and Membrane Protrusions in Developmental Signaling.Journal of developmental biology · 2022Review
- A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesis.Journal of cell science · 2022Article
- Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish.eLife · 2022Article
- Beyond Neurons: Long Distance Communication in Development and Cancer.Frontiers in cell and developmental biology · 2021Review
- Regulation of Wnt Signaling Pathways at the Plasma Membrane and Their Misregulation in Cancer.Frontiers in cell and developmental biology · 2021Review
- The Emerging Mechanisms of Wnt Secretion and Signaling in Development.Frontiers in cell and developmental biology · 2021Review
- Modeling of Wnt-mediated tissue patterning in vertebrate embryogenesis.PLoS computational biology · 2020Article
- Protrusion-Mediated Signaling Regulates Patterning of the Developing Nervous System.Frontiers in cell and developmental biology · 2020Review
- More Favorable Palmitic Acid Over Palmitoleic Acid Modification of Wnt3 Ensures Its Localization and Activity in Plasma Membrane Domains.Frontiers in cell and developmental biology · 2019Article
- Emerging role of contact-mediated cell communication in tissue development and diseases.Histochemistry and cell biology · 2018Review
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- Article
- Direct visualization of the Wntless-induced redistribution of WNT1 in developing chick embryos.Developmental biology · 2018Article
- From top to bottom: Cell polarity in Hedgehog and Wnt trafficking.BMC biology · 2018Review
- The function of endocytosis in Wnt signaling.Cellular and molecular life sciences : CMLS · 2018Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWnt proteins are conserved signaling molecules that regulate pattern formation during animal development. Many Wnt proteins are post-translationally modified by addition of lipid adducts. Wnt8a provides a crucial signal for patterning the anteroposterior axis of the developing neural plate in vertebrates. However, it is not clear how this protein propagates from its source, the blastoderm margin, to the target cells in the prospective neural plate, and how lipid-modifications might influence Wnt8a propagation and activity.
resultsWe have dynamically imaged biologically active, fluorescently tagged Wnt8a in living zebrafish embryos. We find that Wnt8a localizes to membrane-associated, punctate structures in live tissue. In Wnt8a expressing cells, these puncta are found on filopodial cellular processes, from where the protein can be released. In addition, Wnt8a is found colocalized with Frizzled receptor-containing clusters on signal receiving cells. Combining in vitro and in vivo assays, we compare the roles of conserved Wnt8a residues in cell and non-cell-autonomous signaling activity and secretion. Non-signaling Wnt8 variants show these residues can regulate Wnt8a distribution in producing cell membranes and filopodia as well as in the receiving tissue.
conclusionsTogether, our results show that Wnt8a forms dynamic clusters found on filopodial donor cell and on signal receiving cell membranes. Moreover, they demonstrate a differential requirement of conserved residues in Wnt8a protein for distribution in producing cells and receiving tissue and signaling activity during neuroectoderm patterning.
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