ArticleNucleic acids research1987
A transcriptional activator is located in the coding region of the yeast PGK gene.
Article in Nucleic acids research, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity.BMC biology · 2022Article
- Gcn4 Binding in Coding Regions Can Activate Internal and Canonical 5' Promoters in Yeast.Molecular cell · 2018Article
- Construction of lactose-consuming Saccharomyces cerevisiae for lactose fermentation into ethanol fuel.Journal of industrial microbiology & biotechnology · 2013Article
- Identification and partial characterization of extracellular aspartic protease genes from Metschnikowia pulcherrima IWBT Y1123 and Candida apicola IWBT Y1384.Applied and environmental microbiology · 2012Article
- Review
- A downstream regulatory element located within the coding sequence mediates autoregulated expression of the yeast fatty acid synthase gene FAS2 by the FAS1 gene product.Nucleic acids research · 2001Article
- Are all DNA binding and transcription regulation by an activator physiologically relevant?Molecular and cellular biology · 2001Article
- Rare codons are not sufficient to destabilize a reporter gene transcript in tobacco.Plant molecular biology · 1997Article
- Identification and characterisation of two transcriptional repressor elements within the coding sequence of the Saccharomyces cerevisiae HXK2 gene.Nucleic acids research · 1996Article
- The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.Current genetics · 1996Article
- Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae.Microbiological reviews · 1996Review
- Isolation and analysis of the yeast TEA1 gene, which encodes a zinc cluster Ty enhancer-binding protein.Molecular and cellular biology · 1996Article
- Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae).Current genetics · 1995Review
- Transcriptional control of the Saccharomyces cerevisiae ADH1 gene by autonomously replicating sequence binding factor 1.Current microbiology · 1995Article
- The glucose-dependent transactivation activity of ABF1 on the expression of the TDH3 gene in yeast.Current genetics · 1995Article
- Yeast intragenic transcriptional control: activation and repression sites within the coding region of the Saccharomyces cerevisiae LPD1 gene.Molecular and cellular biology · 1994Article
- A small segment of the MAT alpha 1 transcript promotes mRNA decay in Saccharomyces cerevisiae: a stimulatory role for rare codons.Molecular and cellular biology · 1993Article
- Downstream activating sequence within the coding region of a yeast gene: specific binding in vitro of RAP1 protein.Molecular & general genetics : MGG · 1992Article
- Functional analysis of the expression of the 3'-phosphoglycerate kinase pgk gene in Aspergillus nidulans.Molecular & general genetics : MGG · 1992Article
- G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae.Current genetics · 1990Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of heterologous genes from the PGK promoter on high copy number plasmids in yeast is relatively poor compared to the intact PGK gene because of low steady-state RNA levels. In this paper we show that low levels of heterologous RNA are not due to instability of mRNA but result from inefficient transcription due to a defect in RNA synthesis. A comparison of RNA levels from homologous and heterologous transcription units allowed the identification of a positive activator for transcription within the PGK coding region which is required for efficient expression of the PGK gene. Deletion of this region, the "downstream activator sequence", causes a six to ten fold reduction in transcriptional efficiency from the PGK 5' noncoding region.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.