Evidence map›Paper›PMID 24426102›Full record

ArticleIndian journal of microbiology2013

Participation of the Cytoskeletal and Lysosomal Compartments in Campylobacter jejuni Invasion of Caco-2 cells, the Cellular Response by Morphometric Analysis and the Presence of Cytokine and Chemokine Transcripts.

B B Fonseca, I L Santos, D A Rossi, R T Melo, T G Araújo, C U Vieira, E P Mendonça, M E Beletti

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Indian journal of microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

B B FonsecaElectronic Microscopy Centre at the Institute of Biomedical Sciences, Universidade Federal de Uberlândia, Uberlândia-MG, Brazil ; Applied Animal Biotechnology Laboratory at the School of Veterinary Science, Universidade Federal de Uberlândia, Uberlândia-MG, Brazil ; Instituto de Ciências Biomédicas, Rua Ceará, sem número, segundo andar, sala 23, bloco 2D, Campus Umuarama, CEP 38400-902 Uberlândia-MG, Brazil.
I L SantosApplied Animal Biotechnology Laboratory at the School of Veterinary Science, Universidade Federal de Uberlândia, Uberlândia-MG, Brazil.
D A RossiApplied Animal Biotechnology Laboratory at the School of Veterinary Science, Universidade Federal de Uberlândia, Uberlândia-MG, Brazil.
R T MeloApplied Animal Biotechnology Laboratory at the School of Veterinary Science, Universidade Federal de Uberlândia, Uberlândia-MG, Brazil.
T G AraújoGenetics Laboratory of the Institute of Genetics and Biochemistry, The Universidade Federal de Uberlâdia, Uberlândia-MG, Brazil.
C U VieiraGenetics Laboratory of the Institute of Genetics and Biochemistry, The Universidade Federal de Uberlâdia, Uberlândia-MG, Brazil.
E P MendonçaApplied Animal Biotechnology Laboratory at the School of Veterinary Science, Universidade Federal de Uberlândia, Uberlândia-MG, Brazil.
M E BelettiElectronic Microscopy Centre at the Institute of Biomedical Sciences, Universidade Federal de Uberlândia, Uberlândia-MG, Brazil.
Universidade Federal de Uberlândia · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to evaluate the participation of actin and tubulin in the process of internalisation, the interaction of bacterial phagosomes with lysosomes, the morphometric changes and the expression of inflammatory cytokines in Caco-2 cells infected with Campylobacter jejuni. Both actin and tubulin participated in the process of internalisation. Inside the cells, lysosomes fuse with phagosomes, which may lead to bacterial death because after 2 h, the bacteria were not detected by Transmission electron microscopy (TEM). There is increased expression of TGF-β3 during the early stages, and IL-8 was expressed after 60 min p.i. This work showed that C. jejuni invades and causes major morphometric changes in epithelial cells. In response, the cells increase their expression of cytokines that can lead to inflammation. The mechanisms of invasion are dependent on actin and tubulin, and once internalised, lysosomes fuse with phagosomes.

Indexed as

CytoskeletonIL-8InvasionLysosomesTGF-β3

Identifiers

PMID24426102
PMCPMC3626957
OpenAlexW1988580667

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.