ArticleEnvironmental health perspectives2014
Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples.
Article in Environmental health perspectives, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 64 citations in OpenAlex.
- Suppression of brain aromatase decreases the expression of dopaminergic neuron in early development of zebrafish.Fish physiology and biochemistry · 2025Article
- Loss of adult visual responses by developmental BPA exposure is correlated with altered estrogenic signaling.Communications biology · 2025Article
- A novel ERβ high throughput microscopy platform for testing endocrine disrupting chemicals.Heliyon · 2024Article
- Application of Transgenic Zebrafish Models for Studying the Effects of Estrogenic Endocrine Disrupting Chemicals on Embryonic Brain Development.Frontiers in pharmacology · 2022Review
- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- Zebrafish disease models in drug discovery: from preclinical modelling to clinical trials.Nature reviews. Drug discovery · 2021Review
- Health Effects and Life Stage Sensitivities in Zebrafish Exposed to an Estrogenic Wastewater Treatment Works Effluent.Frontiers in endocrinology · 2021Article
- Differential activity of BPA, BPAF and BPC on zebrafish estrogen receptors in vitro and in vivo.Toxicology and applied pharmacology · 2019Article
- Cardiovascular Effects and Molecular Mechanisms of Bisphenol A and Its Metabolite MBP in Zebrafish.Environmental science & technology · 2019Article
- Glucocorticoid deficiency causes transcriptional and post-transcriptional reprogramming of glutamine metabolism.EBioMedicine · 2018Article
- G Protein-Coupled Estrogen Receptor Is Not Required for Sex Determination or Ovary Function in Zebrafish.Endocrinology · 2018Article
- Assaying uptake of endocrine disruptor compounds in zebrafish embryos and larvae.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2018Article
- The Primodos components Norethisterone acetate and Ethinyl estradiol induce developmental abnormalities in zebrafish embryos.Scientific reports · 2018Article
- Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish.Scientific reports · 2018Article
- G protein-coupled estrogen receptor regulates embryonic heart rate in zebrafish.PLoS genetics · 2017Article
- Review
- Quantification of Estradiol Uptake in Zebrafish Embryos and Larvae.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Zebrafish in Toxicology and Environmental Health.Current topics in developmental biology · 2017Review
- ZEBRAFISH AS AN IN VIVO MODEL FOR SUSTAINABLE CHEMICAL DESIGN.Green chemistry : an international journal and green chemistry resource : GC · 2016Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundEnvironmental endocrine disruptors (EEDs) are exogenous chemicals that mimic endogenous hormones such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ERs) in the larval heart compared with the liver. However, it was not known whether the transgenic zebrafish reporter was sensitive enough to detect estrogens from environmental samples, whether environmental estrogens would exhibit tissue-specific effects similar to those of BPA and genistein, or why some compounds preferentially target receptors in the heart.
methodsWe tested surface water samples using a transgenic zebrafish reporter with tandem estrogen response elements driving green fluorescent protein expression (5xERE:GFP). Reporter activation was colocalized with tissue-specific expression of ER genes by RNA in situ hybridization.
resultsWe observed selective patterns of ER activation in transgenic fish exposed to river water samples from the Mid-Atlantic United States, with several samples preferentially activating receptors in embryonic and larval heart valves. We discovered that tissue specificity in ER activation was due to differences in the expression of ER subtypes. ERα was expressed in developing heart valves but not in the liver, whereas ERβ2 had the opposite profile. Accordingly, subtype-specific ER agonists activated the reporter in either the heart valves or the liver.
conclusionThe use of 5xERE:GFP transgenic zebrafish revealed an unexpected tissue-specific difference in the response to environmentally relevant estrogenic compounds. Exposure to estrogenic EEDs in utero was associated with adverse health effects, with the potentially unanticipated consequence of targeting developing heart valves.
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