ArticleJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism2014
Clot injection technique affects thrombolytic efficacy in a rat embolic stroke model: implications for translaboratory collaborations.
Article in Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 12 citations in OpenAlex.
- Feasibility of a Multi-Laboratory Model of Middle Cerebral Artery Thromboembolic Stroke with Thrombolysis: TE-MCAo.Translational stroke research · 2026Article
- Modeling of Cerebral Ischemic Stroke in Conscious Rats via Arterial Air Embolization.Bulletin of experimental biology and medicine · 2024Article
- A Novel Mouse Model for Cerebral Venous Sinus Thrombosis.Translational stroke research · 2021Article
- A review of experimental models of focal cerebral ischemia focusing on the middle cerebral artery occlusion model.F1000Research · 2021Review
- Prevalence of a Multiple Territory Stroke Pattern After Intravenous Thrombolysis.Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association · 2020Observational
- A Canine Model of Hemorrhagic Transformation Using Recombinant Tissue Plasminogen Activator Administration After Acute Ischemic Stroke.Frontiers in neurology · 2019Article
- The IMPROVE Guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments).Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Review
- A novel atherothrombotic model of ischemic stroke induced by injection of collagen into the cerebral vasculature.Journal of neuroscience methods · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Current recommendations encourage the use of embolic stroke (ES) models and replication of results across laboratories in preclinical research. Since such endeavors employ different surgeons, we sought to ascertain the impact of injection technique on outcome and response to thrombolysis in an ES model. Embolic stroke was induced in Male Wistar Kyoto rats (n=166) by a fast or a slow clot injection (CI) technique. Saline or recombinant tissue plasminogen activator (rtPA) was given at 1 hour after stroke. Flow rate curves were assessed in 24 animals. Cerebral perfusion was assessed using laser Doppler flowmetry. Edema corrected infarct volume, hemispheric swelling, hemorrhagic transformation, and neurologic outcome were assessed at 24 hours after stroke. Clot burden was estimated in a subset of animals (n=40). Slow CI resulted in significantly smaller infarct volumes (P=0.024) and better neurologic outcomes (P=0.01) compared with fast CI at 24 hours. Unexpectedly, rtPA treatment attenuated infarct size in fast (P<0.001) but not in slow CI experiments (P=0.382), possibly related to reperfusion injury as indicated by greater hemorrhagic transformation (P<0.001) and hemispheric swelling (P<0.05). Outcome and response to thrombolysis after ES are operator dependent, which needs to be considered when comparing results obtained from different laboratories.
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