Evidence map›Paper›PMID 24424267›Full record

ArticleCytotherapy2014

Human adipose-derived mesenchymal stromal cell pigment epithelium-derived factor cytotherapy modifies genetic and epigenetic profiles of prostate cancer cells.

Olga Zolochevska, Joseph Shearer, Jayne Ellis, Valentina Fokina, Forum Shah, Jeffrey M Gimble, Marxa L Figueiredo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Olga ZolochevskaDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA.
Joseph ShearerDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA.
Jayne EllisDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA.
Valentina FokinaDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA.
Forum ShahPennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
Jeffrey M GimblePennington Biomedical Research Center, Baton Rouge, Louisiana, USA.
Marxa L FigueiredoDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, USA. Electronic address: mlfiguei@utmb.edu.
The University of Texas Medical Branch at Galveston · USPennington Biomedical Research Center · US

Funding

Molecular Mechanisms of Environmental InjuryT32ES007254 · NIEHS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI AMEREDES, BILL T · 1990 to 2022
$6.6M
Preventing prostate cancer progression by ASC delivery of PEDF and IFNbR21CA153165 · NCI · UNIVERSITY OF TEXAS MED BR GALVESTON · PI FIGUEIREDO, MARXA L · 2012 to 2013
$428k
NCI NIH HHS R21 CA153165NCI NIH HHS R21CA153165NIEHS NIH HHS T32 ES007254NIEHS NIH HHS T32ES007254
6 · The paper itself

Abstract

BACKGROUND

aimsAdipose-derived mesenchymal stromal cells (ASCs) are promising tools for delivery of cytotherapy against cancer. However, ASCs can exert profound effects on biological behavior of tumor cells. Our study aimed to examine the influence of ASCs on gene expression and epigenetic methylation profiles of prostate cancer cells as well as the impact of expressing a therapeutic gene on modifying the interaction between ASCs and prostate cancer cells.

methodsASCs were modified by lentiviral transduction to express either green fluorescent protein as a control or pigment epithelium-derived factor (PEDF) as a therapeutic molecule. PC3 prostate cancer cells were cultured in the presence of ASC culture-conditioned media (CCM), and effects on PC3 or DU145. Ras cells were examined by means of real-time quantitative polymerase chain reaction, EpiTect methyl prostate cancer-focused real-time quantitative polymerase chain reaction arrays, and luciferase reporter assays.

resultsASCs transduced with lentiviral vectors were able to mediate expression of several tumor-inhibitory genes, some of which correlated with epigenetic methylation changes on cocultured PC3 prostate cancer cells. When PC3 cells were cultured with ASC-PEDF CCM, we observed a shift in the balance of gene expression toward tumor inhibition, which suggests that PEDF reduces the potential tumor-promoting activity of unmodified ASCs.

conclusionsThese results suggest that ASC-PEDF CCM can promote reprogramming of tumor cells in a paracrine manner. An improved understanding of genetic and epigenetic events in prostate cancer growth in response to PEDF paracrine therapy would enable a more effective use of ASC-PEDF, with the goal of achieving safer yet more potent anti-tumor effects.

Indexed as

Cell- and Tissue-Based TherapyAdipose TissueCarcinogenesisDNA MethylationEpigenesis, GeneticEye ProteinsHumansMaleMesenchymal Stem CellsNerve Growth FactorsPigment Epithelium-Derived FactorProstatic NeoplasmsSerpinsStem Cell NicheTranscriptomeTransgenesEye ProteinsNerve Growth FactorsPigment Epithelium-Derived FactorSerpinsadipose-derived mesenchymal stromal cellsepigeneticsgene expressionpigment epithelium–derived factorprostate cancer

Identifiers

PMID24424267
PMCPMC3951468
OpenAlexW2043389410

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.