ArticleExperimental eye research2014
Thiol-dependent antioxidant activity of interphotoreceptor retinoid-binding protein.
Article in Experimental eye research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 23 citations in OpenAlex.
- RGR-Mediated Photopic Visual Cycle and Oxidative Stress: Potential Mechanisms for Cone Vision Impairment and Retinal Degeneration in Retinitis Pigmentosa Linked to D1080N-IRBP.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Retinol-binding protein 3 in ophthalmology: current evidence, research progress, and future perspectives.Frontiers in immunology · 2026Review
- CryoEM structure and small-angle X-ray scattering analyses of porcine retinol-binding protein 3.Open biology · 2025Article
- Resilience to diabetic retinopathy.Progress in retinal and eye research · 2024Review
- Manifestation of Pathology in Animal Models of Diabetic Retinopathy Is Delayed from the Onset of Diabetes.International journal of molecular sciences · 2024Review
- The Slow Progression of Diabetic Retinopathy Is Associated with Transient Protection of Retinal Vessels from Death.International journal of molecular sciences · 2023Article
- Towards a New Biomarker for Diabetic Retinopathy: Exploring RBP3 Structure and Retinoids Binding for Functional Imaging of Eyes In Vivo.International journal of molecular sciences · 2023Review
- Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-International journal of molecular sciences · 2022Article
- Inhibiting the activation of MAPK (ERK1/2) in stressed Müller cells prevents photoreceptor degeneration.Theranostics · 2022Article
- Interphotoreceptor Retinol-Binding Protein Ameliorates Diabetes-Induced Retinal Dysfunction and Neurodegeneration Through Rhodopsin.Diabetes · 2021Article
- Single particle cryo-EM of the complex between interphotoreceptor retinoid-binding protein and a monoclonal antibody.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Development of a therapeutic anti-HtrA1 antibody and the identification of DKK3 as a pharmacodynamic biomarker in geographic atrophy.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Interphotoreceptor Retinoid-Binding Protein (IRBP) in Retinal Health and Disease.Frontiers in cellular neuroscience · 2020Review
- Simvastatin protects photoreceptors from oxidative stress induced by all-trans-retinal, through the up-regulation of interphotoreceptor retinoid binding protein.British journal of pharmacology · 2019Article
- Visual cycle proteins: Structure, function, and roles in human retinal disease.The Journal of biological chemistry · 2018Article
- Interphotoreceptor retinoid-binding protein removes all-The Journal of biological chemistry · 2017Article
- Fold conservation and proteolysis in zebrafish IRBP structure: Clues to possible enzymatic function?Experimental eye research · 2016Article
- Interphotoreceptor Retinoid-Binding Protein Mitigates Cellular Oxidative Stress and Mitochondrial Dysfunction Induced by All-trans-Retinal.Investigative ophthalmology & visual science · 2016Article
- Technical brief: Pump-probe paradigm in an integrating cavity to study photodecomposition processes.Molecular vision · 2016Article
- Structure of zebrafish IRBP reveals fatty acid binding.Experimental eye research · 2015Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Interphotoreceptor retinoid-binding protein (IRBP), which is critical to photoreceptor survival and function, is comprised of homologous tandem modules each ∼300 amino acids, and contains 10 cysteines, possibly 8 as free thiols. Purification of IRBP has historically been difficult due to aggregation, denaturation and precipitation. Our observation that reducing agent 1,4-dithiothreitol dramatically prevents aggregation prompted investigation of possible functions for IRBP's free thiols. Bovine IRBP (bIRBP) was purified from retina saline washes by a combination of concanavalin A, ion exchange and size exclusion chromatography. Antioxidant activity of the purified protein was measured by its ability to inhibit oxidation of 2,2'-azinobis [3-ethylbenzothiazoline-6-sulfonate] by metmyoglobin. Homology modeling predicted the relationship of the retinoid binding sites to cysteine residues. As a free radical scavenger, bIRBP was more active than ovalbumin, thioredoxin, and vitamin E analog Trolox. Alkylation of free cysteines by N-ethylmaleimide inhibited bIRBP's antioxidant activity, but not its ability to bind all-trans retinol. Structural modeling predicted that Cys 1051 is at the mouth of the module 4 hydrophobic ligand-binding site. Its free radical scavenging activity points to a new function for IRBP in defining the redox environment in the subretinal space.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.