ArticleJournal of cell science2014
Characterization and regulation of an additional actin-filament-binding site in large isoforms of the stereocilia actin-bundling protein espin.
Article in Journal of cell science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.The Journal of cell biology · 2026Article
- The actin cytoskeleton in hair bundle development and hearing loss.Hearing research · 2023Review
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- MicroRNA Signature and Cellular Characterization of Undifferentiated and Differentiated House Ear Institute-Organ of Corti 1 (HEI-OC1) Cells.Journal of the Association for Research in Otolaryngology : JARO · 2022Article
- Espin overexpression causes stereocilia defects and provides an anti-capping effect on actin polymerization.Cytoskeleton (Hoboken, N.J.) · 2022Article
- Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.Cell reports · 2021Article
- Putative Coiled-Coil Domain-Dependent Autoinhibition and Alternative Splicing Determine SHTN1's Actin-Binding Activity.Journal of molecular biology · 2020Article
- Article
- Autosomal dominant Alport syndrome due to a COL4A4 mutation with an additional ESPN variant detected by whole-exome analysis.CEN case reports · 2020Article
- Uncoordinated maturation of developing and regenerating postnatal mammalian vestibular hair cells.PLoS biology · 2019Article
- The stable actin core of mechanosensory stereocilia features continuous turnover of actin cross-linkers.Molecular biology of the cell · 2018Article
- Inframe deletion of humanJournal of medical genetics · 2018Article
- Lhx1/5 control dendritogenesis and spine morphogenesis of Purkinje cells via regulation of Espin.Nature communications · 2017Article
- Stereocilia morphogenesis and maintenance through regulation of actin stability.Seminars in cell & developmental biology · 2017Review
- Article
- Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like.Nature communications · 2016Article
- Article
- Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.The Journal of cell biology · 2016Article
- Live-cell imaging of actin dynamics reveals mechanisms of stereocilia length regulation in the inner ear.Nature communications · 2015Article
- Length regulation of mechanosensitive stereocilia depends on very slow actin dynamics and filament-severing proteins.Nature communications · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The espin actin-bundling proteins, which are produced as isoforms of different sizes from a single gene, are required for the growth of hair cell stereocilia. We have characterized an additional actin-filament-binding site present in the extended amino-termini of large espin isoforms. Constitutively active in espin 2, the site increased the size of actin bundles formed in vitro and inhibited actin fluorescence recovery in microvilli. In espin 1, which has an N-terminal ankyrin repeat domain, the site was autoinhibited by binding between the ankyrin repeat domain and a peptide near the actin-binding site. Deletion of this peptide from espin 1 activated its actin-binding site. The peptide resembled tail homology domain I of myosin III, a ligand of the ankyrin repeat domain localized with espin 1 at the tip of stereocilia. A myosin III tail homology domain I peptide, but not scrambled control peptides, inhibited internal binding of the ankyrin repeat domain and released the espin 1 actin-binding site from autoinhibition. Thus, this regulation could result in local activation of the additional actin-binding site of espin 1 by myosin III in stereocilia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.