ArticleThe Journal of clinical endocrinology and metabolism2014
Transcriptome profiling reveals differentially expressed transcripts between the human adrenal zona fasciculata and zona reticularis.
Article in The Journal of clinical endocrinology and metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 67 citations in OpenAlex.
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- POLYCYSTIC OVARY SYNDROME: ORIGINS AND IMPLICATIONS: The significance of functional adrenal hyperandrogenism in polycystic ovary syndrome across the lifespan.Reproduction (Cambridge, England) · 2025Review
- Human Gonads Do Not Contribute to the Circulating Pool of 11-Oxygenated Androgens.The Journal of clinical endocrinology and metabolism · 2025Article
- Effect of Treatment on Steroidome in Women with Multiple Sclerosis.International journal of molecular sciences · 2025Article
- Altered Steroidome in Women with Multiple Sclerosis.International journal of molecular sciences · 2024Article
- Steroidomics in Men with Schizophrenia.International journal of molecular sciences · 2024Article
- Single-nucleus and spatial transcriptome reveal adrenal homeostasis in normal and tumoural adrenal glands.Clinical and translational medicine · 2024Article
- Steroids-producing nodules: a two-layered adrenocortical nodular structure as a precursor lesion of cortisol-producing adenoma.EBioMedicine · 2024Article
- An integrated single-cell analysis of human adrenal cortex development.JCI insight · 2023Article
- Models of Congenital Adrenal Hyperplasia for Gene Therapies Testing.International journal of molecular sciences · 2023Review
- Inclusion of 11-Oxygenated Androgens in a Clinical Routine LC-MS/MS Setup for Steroid Hormone Profiling.International journal of molecular sciences · 2022Article
- 11-Oxygenated androgens are not secreted by the human ovary: in-vivo data from four different cases of hyperandrogenism.European journal of endocrinology · 2022Article
- Characterization of Aldosterone-producing Cell Cluster (APCC) at Single-cell Resolution.The Journal of clinical endocrinology and metabolism · 2022Article
- Ectopic localization of CYP11B1 and CYP11B2-expressing cells in the normal human adrenal gland.PloS one · 2022Article
- Normal and Premature Adrenarche.Endocrine reviews · 2021Review
- Targeted RNA sequencing of adrenal zones using immunohistochemistry-guided capture of formalin-fixed paraffin-embedded tissue.Molecular and cellular endocrinology · 2021Article
- The Age-Dependent Changes of the Human Adrenal Cortical Zones Are Not Congruent.The Journal of clinical endocrinology and metabolism · 2021Article
- Nonclassic Congenital Adrenal Hyperplasia: What Do Endocrinologists Need to Know?Endocrinology and metabolism clinics of North America · 2021Review
- Morphologic and Molecular Characterization of Adrenals and Adrenal Rest Affected by Congenital Adrenal Hyperplasia.Frontiers in endocrinology · 2021Article
- Non-Canonical Effects of ACTH: Insights Into Adrenal Insufficiency.Frontiers in endocrinology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
contextThe human adrenal zona fasciculata (ZF) and zona reticularis (ZR) are responsible for the production of cortisol and 19-carbon steroids (often called adrenal androgens), respectively. However, the gene profiles and exact molecular mechanisms leading to the functional phenotype of the ZF and ZR are still not clearly defined. In the present study, we identified the transcripts that are differentially expressed in the ZF and ZR.
objectiveThe objective of the study was to compare the transcriptome profiles of ZF and ZR. DESIGN AND
methodsZF and ZR were microdissected from 10 human adrenals. Total RNA was extracted from 10 ZF/ZR pairs and hybridized to Illumina microarray chips. The 10 most differentially expressed transcripts were studied with quantitative RT-PCR (qPCR). Immunohistochemistry was also performed on four zone-specific genes.
resultsMicroarray results demonstrated that only 347 transcripts of the 47 231 were significantly different by 2-fold or greater in the ZF and ZR. ZF had 195 transcripts with 2-fold or greater increase compared with its paired ZR, whereas ZR was found to have 152 transcripts with 2-fold or greater higher expression than in ZF. Microarray and qPCR analysis of transcripts encoding steroidogenic enzymes (n = 10) demonstrated that only 3β-hydroxysteroid dehydrogenase, steroid sulfotransferase, type 5 17β-hydroxysteroid dehydrogenase, and cytochrome b5 were significantly different. Immunohistochemistry and qPCR studies confirmed that the ZF had an increased expression of lymphoid enhancer-binding factor 1 and nephroblastoma overexpressed, whereas ZR showed an increased expression of solute carrier family 27 (fatty acid transporter) (SLC27A2), member 2 and TSPAN12 (tetraspanin 12)
conclusionMicroarray revealed several novel candidate genes for elucidating the molecular mechanisms governing the ZF and ZR, thereby increasing our understanding of the functional zonation of these two adrenocortical zones.
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