ArticlePloS one2014
Bacteria localization and chorion thinning among preterm premature rupture of membranes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- Fetal membrane imaging and the prediction of preterm birth: a systematic review, current issues, and future directions.BMC pregnancy and childbirth · 2016Pooled it
- Mechanisms of vaginal bacteria-induced fetal membrane damage and tocilizumab's therapeutic potential in PPROM.BMC pregnancy and childbirth · 2026Article
- Vaginal colonization, vertical transmission rate, antimicrobial susceptibility profile, and associated factors of potential neonatal pathogens among pregnant women at public health facilities of Northeast Ethiopia.Frontiers in public health · 2025Article
- Biophysical Characterization of a Novel Tri-Layer Placental Allograft Membrane.Advances in wound care · 2022Article
- Alterations in the vaginal microbiota of patients with preterm premature rupture of membranes.Frontiers in cellular and infection microbiology · 2022Article
- The Inflammatory Milieu of Amniotic Fluid Increases with Chorio-Deciduitis Grade in Inflammation-Restricted to Choriodecidua, but Not Amnionitis, of Extra-Placental Membranes.Journal of clinical medicine · 2021Article
- Current and Emerging Strategies for Prediction and Diagnosis of Prelabour Rupture of the Membranes: A Narrative Review.The Malaysian journal of medical sciences : MJMS · 2021Review
- Vaginal microbiome analysis of healthy women during different periods of gestation.Bioscience reports · 2020Observational
- Lactobacilli-dominated cervical microbiota in women with preterm prelabor rupture of membranes.Pediatric research · 2020Article
- Emerging Priorities for Microbiome Research.Frontiers in microbiology · 2020Review
- Toxicant Disruption of Immune Defenses: Potential Implications for Fetal Membranes and Pregnancy.Frontiers in physiology · 2020Article
- The Interplay Between Reproductive Tract Microbiota and Immunological System in Human Reproduction.Frontiers in immunology · 2020Review
- Recent Updates on Research Models and Tools to Study Virus-Host Interactions at the Placenta.Viruses · 2019Review
- Establishment of vaginal microbiota composition in early pregnancy and its association with subsequent preterm prelabor rupture of the fetal membranes.Translational research : the journal of laboratory and clinical medicine · 2019Article
- Roles of Progesterone Receptor Membrane Component 1 in Oxidative Stress-Induced Aging in Chorion Cells.Reproductive sciences (Thousand Oaks, Calif.) · 2019Article
- Article
- Enrichment of Clinically Relevant Organisms in Spontaneous Preterm-Delivered Placentas and Reagent Contamination across All Clinical Groups in a Large Pregnancy Cohort in the United Kingdom.Applied and environmental microbiology · 2018Article
- Infection-induced thrombin production: a potential novel mechanism for preterm premature rupture of membranes (PPROM).American journal of obstetrics and gynecology · 2018Article
- Vaginal dysbiosis increases risk of preterm fetal membrane rupture, neonatal sepsis and is exacerbated by erythromycin.BMC medicine · 2018Article
- The oral microbiome and adverse pregnancy outcomes.International journal of women's health · 2017Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveBacterial colonization of the fetal membranes and its role in pathogenesis of membrane rupture is poorly understood. Prior retrospective work revealed chorion layer thinning in preterm premature rupture of membranes (PPROM) subjects. Our objective was to prospectively examine fetal membrane chorion thinning and to correlate to bacterial presence in PPROM, preterm, and term subjects. STUDY
designPaired membrane samples (membrane rupture and membrane distant) were prospectively collected from: PPROM = 14, preterm labor (PTL = 8), preterm no labor (PTNL = 8), term labor (TL = 10), and term no labor (TNL = 8), subjects. Sections were probed with cytokeratin to identify fetal trophoblast layer of the chorion using immunohistochemistry. Fluorescence in situ hybridization was performed using broad range 16 s ribosomal RNA probe. Images were evaluated, chorion and choriodecidua were measured, and bacterial fluorescence scored. Chorion thinning and bacterial presence were compared among and between groups using Student's t-test, linear mixed effect model, and Poisson regression model (SAS Cary, NC).
resultsIn all groups, the fetal chorion cellular layer was thinner at rupture compared to distant site (147.2 vs. 253.7 µm, p<0.0001). Further, chorion thinning was greatest among PPROM subjects compared to all other groups combined, regardless of site sampled [PPROM(114.9) vs. PTL(246.0) vs. PTNL(200.8) vs. TL(217.9) vs. TNL(246.5)]. Bacteria counts were highest among PPROM subjects compared to all other groups regardless of site sampled or histologic infection [PPROM(31) vs. PTL(9) vs. PTNL(7) vs. TL(7) vs. TNL(6)]. Among all subjects at both sites, bacterial counts were inversely correlated with chorion thinning, even excluding histologic chorioamnionitis (p<0.0001 and p = 0.05).
conclusionsFetal chorion was uniformly thinner at rupture site compared to distant sites. In PPROM fetal chorion, we demonstrated pronounced global thinning. Although cause or consequence is uncertain, bacterial presence is greatest and inversely correlated with chorion thinning among PPROM subjects.
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