ArticleMolecular and cellular biology2014
C/EBPa-mediated activation of microRNAs 34a and 223 inhibits Lef1 expression to achieve efficient reprogramming into macrophages.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- The Role of Omega-3 Polyunsaturated Fatty Acids in Modulating Inflammation and Breast Cancer: A Systematic Review of In Vitro, Animal, and Human Studies.Current oncology reports · 2026Pooled it
- Aging-associated DNA methylation of LEF1 modulates inflammation and neurodegenerative pathways.Frontiers in immunology · 2025Article
- Renal Tissue-Derived Exosomal miRNA-34a in Diabetic Nephropathy Induces Renal Tubular Cell Fibrosis by Promoting the Polarization of M1 Macrophages.IET nanobiotechnology · 2024Article
- Review
- Differential gene expression analysis identified determinants of cell fate plasticity during radiation-induced regeneration in Drosophila.PLoS genetics · 2022Article
- Dynamics of alternative splicing during somatic cell reprogramming reveals functions for RNA-binding proteins CPSF3, hnRNP UL1, and TIA1.Genome biology · 2021Article
- Oncogenic RUNX3: A Link between p53 Deficiency and MYC Dysregulation.Molecules and cells · 2020Review
- Osteoclastic microRNAs and their translational potential in skeletal diseases.Seminars in immunopathology · 2019Review
- Long noncoding RNA LEF1-AS1 silencing suppresses the initiation and development of prostate cancer by acting as a molecular sponge of miR-330-5p via LEF1 repression.Journal of cellular physiology · 2019Article
- Cellular plasticity, caspases and autophagy; that which does not kill us, well, makes us different.Open biology · 2018Review
- Basal-like breast cancer: molecular profiles, clinical features and survival outcomes.BMC medical genomics · 2017Article
- C/EBP-Induced Transdifferentiation Reveals Granulocyte-Macrophage Precursor-like Plasticity of B Cells.Stem cell reports · 2017Article
- Role of miR-34a-5p in Hematopoietic Progenitor Cells Proliferation and Fate Decision: Novel Insights into the Pathogenesis of Primary Myelofibrosis.International journal of molecular sciences · 2017Article
- miRNAs in acute myeloid leukemia.Oncotarget · 2017Review
- microRNAs and Acute Myeloid Leukemia Chemoresistance: A Mechanistic Overview.Frontiers in oncology · 2017Review
- Wnt signaling pathway protein LEF1 in cancer, as a biomarker for prognosis and a target for treatment.American journal of cancer research · 2017Review
- Epigenetic Regulation of Innate Immunity by microRNAs.Antibodies (Basel, Switzerland) · 2016Review
- Familial adenomatous patients with desmoid tumours show increased expression of miR-34a in serum and high levels in tumours.Oncoscience · 2016Article
- LEF1 Targeting EMT in Prostate Cancer Invasion Is Regulated by miR-34a.Molecular cancer research : MCR · 2015Article
- miR-17 deregulates a core RUNX1-miRNA mechanism of CBF acute myeloid leukemia.Molecular cancer · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) exert negative effects on gene expression and influence cell lineage choice during hematopoiesis. C/EBPa-induced pre-B cell-to-macrophage transdifferentiation provides an excellent model to investigate the contribution of miRNAs to hematopoietic cell identity, especially because the two cell types involved fall into separate lymphoid and myeloid branches. In this process, efficient repression of the B cell-specific program is essential to ensure transdifferentation and macrophage function. miRNA profiling revealed that upregulation of miRNAs is highly predominant compared with downregulation and that C/EBPa directly regulates several upregulated miRNAs. We also determined that miRNA 34a (miR-34a) and miR-223 sharply accelerate C/EBPa-mediated transdifferentiation, whereas their depletion delays this process. These two miRNAs affect the transdifferentiation efficiency and activity of macrophages, including their lipopolysaccharide (LPS)-dependent inflammatory response. miR-34a and miR-223 directly target and downregulate the lymphoid transcription factor Lef1, whose ectopic expression delays transdifferentiation to an extent similar to that seen with miR-34a and miR-223 depletion. In addition, ectopic introduction of Lef1 in macrophages causes upregulation of B cell markers, including CD19, Pax5, and Ikzf3. Our report demonstrates the importance of these miRNAs in ensuring the erasure of key B cell transcription factors, such as Lef1, and reinforces the notion of their essential role in fine-tuning the control required for establishing cell identity.
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