ArticleThe EMBO journal2014
γ-Tubulin Ring Complexes and EB1 play antagonistic roles in microtubule dynamics and spindle positioning.
Article in The EMBO journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- SAPCD2 phosphorylation modulates astral microtubule stability to control spindle orientation.Communications biology · 2026Article
- Mutation of Tubgcp6 induces hematopoietic stem and progenitor cell exhaustion in zebrafish.Communications biology · 2026Article
- Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis.Nature communications · 2023Article
- CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.BMC biology · 2022Article
- Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis.Nature chemical biology · 2021Article
- Spindle positioning and its impact on vertebrate tissue architecture and cell fate.Nature reviews. Molecular cell biology · 2021Review
- Arl4D-EB1 interaction promotes centrosomal recruitment of EB1 and microtubule growth.Molecular biology of the cell · 2020Article
- Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.Cell death and differentiation · 2020Article
- Roles of end-binding 1 protein and gamma-tubulin small complex in cytokinesis and flagella formation of Giardia lamblia.MicrobiologyOpen · 2019Article
- Microtubule nucleation by γ-tubulin complexes and beyond.Essays in biochemistry · 2018Review
- Regulation of mitotic spindle orientation: an integrated view.EMBO reports · 2016Review
- ASK1 controls spindle orientation and positioning by phosphorylating EB1 and stabilizing astral microtubules.Cell discovery · 2016Article
- γ-Tubulin complexes in microtubule nucleation and beyond.Molecular biology of the cell · 2015Article
- Mutations in TUBGCP4 alter microtubule organization via the γ-tubulin ring complex in autosomal-recessive microcephaly with chorioretinopathy.American journal of human genetics · 2015Article
- Human Nek7-interactor RGS2 is required for mitotic spindle organization.Cell cycle (Georgetown, Tex.) · 2015Article
- End-binding 1 protein overexpression correlates with glioblastoma progression and sensitizes to Vinca-alkaloids in vitro and in vivo.Oncotarget · 2014Article
- TACC3 protein regulates microtubule nucleation by affecting γ-tubulin ring complexes.The Journal of biological chemistry · 2014Article
- CLASPs are required for proper microtubule localization of end-binding proteins.Developmental cell · 2014Article
- Short-circuiting microtubule plus and minus end proteins in spindle positioning.The EMBO journal · 2014Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
γ-Tubulin is critical for microtubule (MT) assembly and organization. In metazoa, this protein acts in multiprotein complexes called γ-Tubulin Ring Complexes (γ-TuRCs). While the subunits that constitute γ-Tubulin Small Complexes (γ-TuSCs), the core of the MT nucleation machinery, are essential, mutation of γ-TuRC-specific proteins in Drosophila causes sterility and morphological abnormalities via hitherto unidentified mechanisms. Here, we demonstrate a role of γ-TuRCs in controlling spindle orientation independent of MT nucleation activity, both in cultured cells and in vivo, and examine a potential function for γ-TuRCs on astral MTs. γ-TuRCs locate along the length of astral MTs, and depletion of γ-TuRC-specific proteins increases MT dynamics and causes the plus-end tracking protein EB1 to redistribute along MTs. Moreover, suppression of MT dynamics through drug treatment or EB1 down-regulation rescues spindle orientation defects induced by γ-TuRC depletion. Therefore, we propose a role for γ-TuRCs in regulating spindle positioning by controlling the stability of astral MTs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.