ArticleThe Journal of biological chemistry2014
Activation of the prereplication complex is blocked by mimosine through reactive oxygen species-activated ataxia telangiectasia mutated (ATM) protein without DNA damage.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 38 citations in OpenAlex.
- Investigating the role of mimosine-induced genotoxic stress through DNA repair profiling.Molecular therapy. Nucleic acids · 2026Article
- PARP1-mediated 5' flap dynamics facilitate Okazaki fragment maturation.bioRxiv : the preprint server for biology · 2026Article
- Evolution of the Defense Compounds Against Biotic Stressors in the Invasive Plant SpeciesMolecules (Basel, Switzerland) · 2025Review
- Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cells.Science advances · 2025Article
- CDK4/6 inhibitor palbociclib promotes SARS-CoV-2 cell entry by down-regulating SKP2 dependent ACE2 degradation.Antiviral research · 2023Article
- CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis.Molecular cell · 2023Article
- Allelopathy and Allelochemicals ofPlants (Basel, Switzerland) · 2022Review
- Genome Replication Is Associated With Release of Immunogenic DNA Waste.Frontiers in immunology · 2022Article
- Protective Effect of Thymidine on DNA Damage Induced by Hydrogen Peroxide in Human Hepatocellular Cancer Cells.ACS omega · 2020Article
- Desuppression of TGF-β signaling via nuclear c-Abl-mediated phosphorylation of TIF1γ/TRIM33 at Tyr-524, -610, and -1048.Oncogene · 2019Article
- S-phase Specific Downregulation of Human ONeoplasia (New York, N.Y.) · 2018Article
- A NuRD Complex from Xenopus laevis Eggs Is Essential for DNA Replication during Early Embryogenesis.Cell reports · 2018Article
- v-Src-driven transformation is due to chromosome abnormalities but not Src-mediated growth signaling.Scientific reports · 2018Article
- Impediment of Replication Forks by Long Non-coding RNA Provokes Chromosomal Rearrangements by Error-Prone Restart.Cell reports · 2017Article
- Nucleoporins redistribute inside the nucleus after cell cycle arrest induced by histone deacetylases inhibition.Nucleus (Austin, Tex.) · 2017Article
- Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols.Journal of visualized experiments : JoVE · 2017Article
- Growth arrest of vascular smooth muscle cells in suspension culture using low-acyl gellan gum.In vitro cellular & developmental biology. Animal · 2017Article
- Protective role for lipid modifications of Src-family kinases against chromosome missegregation.Scientific reports · 2016Article
- Genome-wide identification and characterisation of human DNA replication origins by initiation site sequencing (ini-seq).Nucleic acids research · 2016Article
- DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mimosine is an effective cell synchronization reagent used for arresting cells in late G1 phase. However, the mechanism underlying mimosine-induced G1 cell cycle arrest remains unclear. Using highly synchronous cell populations, we show here that mimosine blocks S phase entry through ATM activation. HeLa S3 cells are exposed to thymidine for 15 h, released for 9 h by washing out the thymidine, and subsequently treated with 1 mM mimosine for a further 15 h (thymidine → mimosine). In contrast to thymidine-induced S phase arrest, mimosine treatment synchronizes >90% of cells at the G1-S phase boundary by inhibiting the transition of the prereplication complex to the preinitiation complex. Mimosine treatment activates ataxia telangiectasia mutated (ATM)/ataxia telangiectasia and Rad3-related (ATR)-mediated checkpoint signaling without inducing DNA damage. Inhibition of ATM activity is found to induce mimosine-arrested cells to enter S phase. In addition, ATM activation by mimosine treatment is mediated by reactive oxygen species (ROS). These results suggest that, upon mimosine treatment, ATM blocks S phase entry in response to ROS, which prevents replication fork stalling-induced DNA damage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.