ArticleThe American journal of tropical medicine and hygiene2014
Molecular mechanisms of in vitro betulin-induced apoptosis of Leishmania donovani.
Article in The American journal of tropical medicine and hygiene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Exploring the Antiparasitic Activity of the Sea Cucumber Isostichopus sp. aff. badionotus From the Northern Coast of Colombia Against Trypanosoma cruzi.TheScientificWorldJournal · 2026Article
- Oxidative Stress and Survival ofExpert reviews in molecular medicine · 2025Review
- Topical Application of Oxylipin (3Journal of natural products · 2025Article
- Oregano essential oilFrontiers in cellular and infection microbiology · 2025Article
- Innovating Leishmaniasis Treatment: A Critical Chemist's Review of Inorganic Nanomaterials.ACS infectious diseases · 2024Review
- Synthesis and characterization of zinc derivatized 3, 5-dihydroxy 4', 7-dimethoxyflavone and its anti leishmaniasis activity against Leishmania donovani.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Article
- Leishmanicidal Activity of Betulin Derivatives inMicroorganisms · 2021Article
- Promastigotes ofCurrent research in parasitology & vector-borne diseases · 2021Article
- Related Pentacyclic Triterpenes Have Immunomodulatory Activity in Chronic Experimental Visceral Leishmaniasis.Journal of immunology research · 2021Article
- Lup-20(29)-en-3β,28-di-yl-nitrooxy acetate affects MCF-7 proliferation through the crosstalk between apoptosis and autophagy in mitochondria.Cell death & disease · 2018Article
- Novel betulin derivative induces anti-proliferative activity by GOncology letters · 2018Article
- Methionine aminopeptidase 2 is a key regulator of apoptotic like cell death in Leishmania donovani.Scientific reports · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although leishmanial infections of humans occur globally, the major health impact lies in developing nations, thus, leishmaniases remain "neglected" diseases for new drugs development. Multidrug resistance has been documented in most countries where leishmaniases is endemic. Betulin is a widely available and affordable natural product exerting leishmanicidal activity at micromolar concentration. In this study, the molecular mechanisms of death that contribute to the anti-leishmanial activity of betulin are investigated. In promastigotes, betulin stimulated reactive oxygen species generation at micromolar concentrations in Leishmania. Apoptosis was observed in betulin-treated promastigotes using flow cytometric analysis of treated cells stained with annexin V-FITC and propidium iodide. Furthermore, betulin treatment of promastigotes led to mitochondrial membrane damage, activation of caspase-like proteases, and DNA fragmentation in Leishmania donovani promastigotes. Betulin treatment of amastigotes cultured within macrophages, resulted in a reduced number of amastigotes, with no substantive cytotoxic damage to the host macrophage cells at leishmanicidal drug concentrations.
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